Fine structure of the canal neuromasts of the lateral line system in the adult zebrafish          下载免费PDF全文

R. Laurà  F. Abbate  G. P. Germanà  G. Montalbano  A. Germanà  M. Levanti 《Anatomia, histologia, embryologia》2018,47(4):322-329

The mechanosensory lateral line system of fish is responsible for several functions such as balance, hearing, and orientation in water flow and is formed by neuromast receptor organs distributed on head, trunk and tail. Superficial and canal neuromasts can be distinguished for localization and morphological differences. Several information is present regarding the superficial neuromasts of zebrafish and other teleosts especially during larval and juvenile stages, while not as numerous data are so far available about the ultrastructural characteristics of the canal neuromasts in adult zebrafish. Therefore, the aim of this study was to investigate by transmission electron microscopy the ultrastructural aspects of cells present in the canal neuromasts. Besides the typical cellular aspects of the neuromast, different cellular types of hair cells were observed that could be identified as developing hair cells during the physiological turnover. The knowledge of the observed cellular types of the canal neuromasts and their origin could give a contribution to studies carried out on adult zebrafish used as model in neurological and non‐neurological damages, such as deafness and vestibular disorders.  相似文献   

斑马鱼幼鱼嗜中性粒细胞对副溶血弧菌清除的动态变化     

郭欣娅  季策  谢子健  季帆  祖尧  任建峰  张庆华 《上海海洋大学学报》2020,29(2):180-188

副溶血弧菌(Vibrio parahaemolyticus)是人-兽-鱼共患的致病菌,严重威胁食品安全和人类的健康。为了研究宿主嗜中性粒细胞清除细菌感染的动态变化过程,利用红色荧光蛋白标记的副溶血弧菌Vp57~(RFP)菌株感染受精后48 h(hours post fertilization,hpf)的斑马鱼(Danio rerio)幼鱼耳泡,建立局部感染模型,并以大肠杆菌(Escherichia coli)Ec01菌株为对照,系统比较了副溶血弧菌和大肠杆菌的半数致死剂量(median lethal dose,LD_(50))、存活曲线以及和嗜中性粒细胞相互作用的动态过程,RT-qPCR验证炎症相关基因il1b和il10在感染前后的表达量变化。结果显示副溶血弧菌和大肠杆菌感染斑马鱼幼鱼的LD_(50)分别为7.90×10~8 CFU/mL和1.14×10~(11) CFU/mL,与感染大肠杆菌相比,斑马鱼感染副溶血弧菌后招募嗜中性粒细胞的速度更快,且数量更多。RT-qPCR结果发现斑马鱼感染副溶血弧菌后在相同时间点能够激活更高的il1b和il10基因的表达,引起强烈的炎症反应。基于以上研究,我们建立的副溶血弧菌-斑马鱼幼鱼耳泡局部感染模型,为进一步研究嗜中性粒细胞清除副溶血弧菌感染的动态过程,以及深入揭示天然免疫应答机制提供了依据。  相似文献   

Oxidative stress inducers potentiate 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated pre-cardiac edema in larval zebrafish     

Katsuki TANAKA  Hikaru ADACHI  Hironobu AKASAKA  Junya TAMAOKI  Yuji FUSE  Makoto KOBAYASHI  Takio KITAZAWA  Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1050

We reported the involvement of oxidative stress and prostaglandins including thromboxane and prostacyclin in pre-cardiac edema (early edema) caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). While the involvement of oxidative stress in TCDD-induced toxicity has been frequently reported, the mechanism of its action is still unclear. In the present study, oxidative stress inducers including paraquat, hydrogen peroxide (H₂O₂) and rotenone augmented early edema (edema) induced by a low concentration of TCDD (0.1 ppb) at 55 hr post fertilization (hpf), while each of them alone did not cause edema. Edema caused by TCDD plus oxidative stress inducers was almost abolished by antioxidants, an antagonist for thromboxane receptor (ICI-192,605) and an agonist for prostacyclin receptor (beraprost), suggesting that the site of action of these inducers was in the regular signaling pathway after activation of aryl hydrocarbon receptor type 2 (AHR2) by TCDD. Oxidative stress inducers also enhanced edema caused by an agonist for the thromboxane receptor (U46619), and the enhancement was also inhibited by antioxidants. Sulforaphane and auranofin, activators of Nrf2 that is a master regulator of anti-oxidative response, did not affect U46619-evoked edema but almost abolished TCDD-induced edema and potentiation by paraquat in both TCDD- and U46619-induced edema. Taken together, the results suggest that oxidative stress augments pre-cardiac edema caused by TCDD via activation of thromboxane receptor-mediated signaling in developing zebrafish. As paraquat and other oxidative stress inducers used also are environmental pollutants, interaction between dioxin-like compounds and exogenous source of oxidative stress should also be considered.  相似文献   

Lipid-based transfection as a method for gene delivery in zebrafish (Danio rerio) embryos     

Vanesa Robles  & M Leonor Cancela 《Aquaculture Research》2007,38(12):1317-1322

A major challenge to the widespread production of transgenic, knockout and knockdown zebrafish has been the absence of a simple and effective procedure for introducing macromolecules into the fertilized egg. None of the existing techniques for gene transfer in fish embryos has proven to be a major advance over cytoplasm microinjection, which is a technically demanding and time‐consuming procedure. This report addresses this need, considering that the development of protocols for lipid‐based transfection with fish embryos would considerably simplify gene transfer in this complex biological model. In this study, lipid‐based transfection with two different reporter vectors was carried out in zebrafish embryos at different developmental stages. The parameters tested included different plasmid/transfection reagent ratios as well as the influence of an added transfection enhancer reagent. When embryos were transfected in the blastula stage with a pEGFP‐N1 vector, more than 35% successfully incorporated the plasmid and expressed the fluorescent protein 24 h after transfection. The transfection enhancer did not show any significant effect in our experiments. This work presents an approach to implement this technique as a faster, cheaper and more practical alternative than microinjection.  相似文献   

Aquatic birnavirus induces apoptosis through activated caspase-8 and -3 in a zebrafish cell line     

Hong JR  Huang LJ  Wu JL 《Journal of fish diseases》2005,28(3):133-140

In this study, the possible influence of temperature on infectious pancreatic necrosis virus (IPNV)-induced apoptosis in a zebrafish liver epithelium (ZLE) cell line was investigated. At a lower temperature (18 degrees C), there was expression of viral proteins VP2 and VP3 at 4 h post-infection (p.i.). At this time no expression was found in the high temperature group at 28 degrees C. The cell survival ratio was 52 and 18% at 24 and 48 h p.i., respectively, during IPNV infection at 18 degrees C. In addition, we assayed for apoptosis in IPNV-infected cells with terminal deoxynucleotidyl transferase (TdT)-mediated end labelling (TUNEL) of DNA at different dosages of virus. We found a ratio of apoptotic cells of 8 and 25% at 12 and 18 h p.i., respectively, in the multiplicity of infection (MOI) 1 group. The MOI 10 group had 20 and 45% apoptotic cells at 12 and 18 h, respectively. Furthermore, at 18 degrees C IPNV activated the caspase-8 and 3 from 1.5 to 2 times at 12 and 18 h p.i., respectively. Taken together, these findings suggest that successful virus replication occurs at the low temperature (18 degrees C) compared with the non-permissive temperature of 28 degrees C. Thus, IPNV replication is capable of activating caspase-8 and -3 and inducing host apoptosis.  相似文献   

Ochracenoids A and B,Guaiazulene-Based Analogues from Gorgonian Anthogorgia ochracea Collected from the South China Sea     

Juan-Juan Zheng  Chang-Lun Shao  Min Chen  Li-She Gan  Yu-Chun Fang  Xu-Hui Wang  Chang-Yun Wang 《Marine drugs》2014,12(3):1569-1579

Two new guaiazulene-based analogues, ochracenoids A (1) and B (2), along with four known analogues (3–6), were isolated from the gorgonian Anthogorgia ochracea collected from the South China Sea. The planar structures of the new compounds were elucidated by comprehensive spectroscopic data. The absolute configuration of 1 was determined as 3R by the comparison of TDDFT calculated electronic circular dichroism with its experimental spectrum. Compound 1 is a rare guaiazulene-based analogue possessing a unique C₁₆ skeleton. The possible generation process of 1 through an intermolecular one-carbon-transfer reaction was also discussed. Compound 2 was previously described as a presumed intermediate involved in the biogenesis of anthogorgienes A and I. Compound 3 exhibited antiproliferative effects on the embryo development of zebrafish Danio rerio.  相似文献   

Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro     

Jun-Xiu Liu  Min-Qi Luo  Meng Xia  Qi Wu  Si-Mei Long  Yaohua Hu  Guang-Chun Gao  Xiao-Li Yao  Mian He  Huanxing Su  Xiong-Ming Luo  Shu-Zhong Yao 《Marine drugs》2014,12(5):2790-2801

Angiogenesis is the formation of blood vessels from pre-existing vasculature. Excessive or uncontrolled angiogenesis is a major contributor to many pathological conditions whereas inhibition of aberrant angiogenesis is beneficial to patients with pathological angiogenesis. Catunaregin is a core of novel marine compound isolated from mangrove associate. The potential anti-angiogenesis of catunaregin was investigated in human umbilical vein endothelial cells (HUVECs) and zebrafish. HUVECs were treated with different concentrations of catunaregin in the presence or absence of VEGF. The angiogenic phenotypes including cell invasion cell migration and tube formation were evaluated following catunaregin treatment in HUVECs. The possible involvement of AKT, eNOS and ERK1/2 in catunaregin-induced anti-angiogenesis was explored using Western blotting. The anti-angiogenesis of catunaregin was further tested in the zebrafish embryo neovascularization and caudal fin regeneration assays. We found that catunaregin dose-dependently inhibited angiogenesis in both HUVECs and zebrafish embryo neovascularization and zebrafish caudal fin regeneration assays. In addition, catunaregin significantly decreased the phosphorylation of Akt and eNOS, but not the phosphorylation of ERK1/2. The present work demonstrates that catunaregin exerts the anti-angiogenic activity at least in part through the regulation of the Akt and eNOS signaling pathways.  相似文献   

斑马鱼在鱼类病原免疫学研究中的应用   总被引：1，自引：0，他引：1  

吴唯维  李盼  柯翎  李素一  陈华  陈叙  林天龙  林晨韬 《福建农业学报》2014,29(6)

斑马鱼全基因组测序已经完成,建立了多个专门的在线数据库,作为模式生物在遗传学、发育生物学等领域已得到了广泛的应用.本文介绍了斑马鱼用于鱼类病原免疫学研究的优点,包括个体发育过程透明、免疫系统较成熟,具有先天性和获得性2种免疫系统;多种鱼类来源的细菌和病毒在成年斑马鱼和胚胎中都能感染和复制,使其成为研究鱼类病原的入侵机制和宿主免疫应答的模式生物.同时,对当前已建立的鱼类细菌和病毒病原斑马鱼感染模型以及在疫苗方面的研究现状作简要概述.  相似文献   

兽药添加剂喹乙醇的生态毒理学效应研究   总被引：6，自引：0，他引：6  

陈海刚  李兆利  徐韵  曲甍甍  孔志明 《农业环境科学学报》2006,25(4):885-889

采用水生生物斑马鱼(Daniorerio)、鲤鱼(Cyprinuscarpio)和土壤生物赤子爱胜蚓(Eiseniafoetida)作为生物材料,通过斑马鱼急性毒性试验、斑马鱼胚胎发育试验、鲤鱼肾细胞单细胞凝胶电泳试验、蚯蚓急性毒性试验、蚯蚓生化毒性试验以及蚯蚓体腔细胞单细胞凝胶电泳试验,对兽药添加剂喹乙醇分别从生物个体、细胞和分子水平进行了生态毒理学效应研究。急性毒性试验结果表明,喹乙醇实验浓度范围内对斑马鱼和蚯蚓的急性毒性均不大。胚胎发育试验结果表明,喹乙醇对斑马鱼胚胎的72hEC50为221.20mg·L-1,具有明显致畸效应。蚯蚓生化毒性试验表明,喹乙醇能促进蚯蚓体内SOD酶活性,并呈现一定剂量-效应关系,但对纤维素酶影响不明显,单细胞凝胶电泳试验结果表明,喹乙醇具有一定遗传毒性,能引起鲤鱼肾脏细胞和蚯蚓体腔细胞DNA损伤,与空白对照相比有显著差异,并呈现出一定剂量-效应关系。  相似文献   

维生素E对毒死蜱胁迫下斑马鱼血清CAT和SOD的保护作用   总被引：1，自引：0，他引：1  

焦铭  孙雪  李笔  黄俊潮 《湖北农业科学》2015,(7)

通过对斑马鱼肌肉注射不同浓度毒死蜱以及维生素E,研究斑马鱼血清中过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活力变化情况以及维生素E的抗氧化作用。试验分为5组,依次为对照组和低、中、高浓度毒死蜱组(0.002、0.02、0.2 mg/kg)以及高浓度联合维生素E(0.2 mg/kg+VE)治疗组。结果表明,毒死蜱不同浓度组短时间(24 h)作用均使两种酶活性升高(P﹤0.01),加维生素E组活性增高更显著;长时间(168 h)作用则明显抑制酶活性(P﹤0.01),维生素E组酶活性变化较平缓。表明毒死蜱对斑马鱼机体抗氧化系统损伤作用明显,维生素E对CAT和SOD活力有保护作用。  相似文献