Various protein and albumin corrections of the serum fructosamine concentration in the diagnosis of canine diabetes mellitus     

A. L. Jensen 《Veterinary research communications》1993,17(1):13-23

Fructosamines are formed when glucose reacts non-enzymatically with amino groups on proteins, and previous studies have indicated that the serum fructosamine concentration could be of importance in the diagnosis of canine diabetes mellitus. Owing to the connection between the protein/albumin concentration and serum fructosamine concentration, it has been suggested that the serum fructosamine concentration should be corrected for the protein/albumin concentration. Thus, the purpose of the present study was to evaluate the uncorrected serum fructosamine concentration and various protein and albumin corrections of the serum fructosamine concentration in the separation of dogs with diabetes mellitus from dogs with other diseases that presented with clinical signs suggestive of diabetes mellitus. The evaluation was assisted by relative operating characteristic curves (ROC curves), which may be used to compare various diagnostic tests under equivalent conditions (equal true positive ratios or false positive ratios) and over the entire range of cutoff values. A total of 58 dogs (15 dogs with diabetes mellitus and 43 dogs with other diseases) were included in the study. Serum fructosamine concentration, serum total protein concentration and serum albumin concentration were measured in each dog, and various corrections of the serum fructosamine concentration for protein or albumin concentration were made. Comparing the ROC curves of the uncorrected and each corrected serum fructosamine concentration indicated that there was no decisive difference between the uncorrected and the corrected serum fructosamine concentrations in discriminating between dogs with and without diabetes mellitus. Hence, correcting the serum fructosamine concentration as a routine procedure cannot be advocated from the results of the study. Moreover, the sensitivity and specificity of the uncorrected serum fructosamine concentration were very high, 0.93 and 0.95, respectively, further evidence of the value of the uncorrected serum fructosamine concentration in the diagnosis of canine diabetes mellitus.Abbreviations SFC serum fructosamine concentration - SFC-P serum fructosamine concentration corrected for the actual serum total protein concentration - SFC-A serum fructosamine concentration corrected for the actual serum albumin concentration - SFC-Po serum fructosamine concentration corrected for the actual serum total protein concentration, only when the serum total protein concentration is outside the reference interval - SFC-Ao serum fructosamine concentration corrected for the actual serum albumin concentration, only when the serum albumin concentration is outside the reference interval - SFC-K serum fructosamine concentration corrected according to Kawamotoet al. (1992)  相似文献   

Modeling susceptibility to deforestation of remaining ecosystems in North Central Mexico with logistic regression     

L. Miranda-Aragón  E.J. Trevi o-Garza  J. Jiménez-Pérez  O.A. Aguirre-Calderón  M.A. González-Tagle  M. Pompa-García  C.A. Aguirre-Salado 《林业研究》2012,23(3):345-354

Determining underlying factors that foster deforestation and delineating forest areas by levels of susceptibility are of the main challenges when defining policies for forest management and planning at regional scale. The susceptibility to deforestation of remaining forest ecosystems (shrubland, temperate forest and rainforest) was conducted in the state of San Luis Potosi, located in north central Mexico. Spatial analysis techniques were used to detect the deforested areas in the study area during 1993-2007. Logistic regression was used to relate explanatory variables (such as social, investment, forest production, biophysical and proximity factors) with susceptibility to deforestation to construct predictive models with two focuses: general and by biogeographical zone.In all models, deforestation has positive correlation with distance to rainfed agriculture, and negative correlation with slope, distance to roads and distance to towns. Other variables were significant in some cases, but in others they had dual relationships, which varied in each biogeographical zone. The results show that the remaining rainforest of Huastecaregion is highly susceptible to deforestation. Both approaches show that more than 70% of the current rainforest area has high and very high levels of susceptibility to deforestation. The values represent a serious concern with global warming whether tree carbon is released to atmosphere. However, after some considerations, encouraging forest environmental services appears to be the best alternative to achieve sustainable forest management.  相似文献   

围产前期奶牛血清相关能量平衡指标和脂肪因子对酮病的预警作用及意义     

肖劲邦  朱颍琨  钱柏霖  邓俊良  彭广能  余树民  左之才  马晓平  钟志军  任志华  周紫峣  刘海峰  曹随忠  沈留红 《中国农业大学学报》2019,24(9):79-87

为探究围产期奶牛产前14和7 d血清能量平衡指标以及脂肪因子对产后酮病的预警作用及意义。从奶牛产前21 d至产后21 d,每隔7 d跟踪采集60头围产期奶牛尾静脉血,最终筛选出健康奶牛21头,酮病奶牛17头,检测奶牛各时间点血清BHBA、NEFA、Glu、TG、INS、 ADP、LEP、抵抗素、IL-6和TNF-α含量,利用二元Logistic回归模型筛选预警酮病的指标,再采用ROC曲线确定各风险评估指标的诊断效果及最佳分界值。结果表明:奶牛产前14 d时,血清BHBA和NEFA可作为酮病的预警指标;BHBA的最佳分界值为0.42 mmol/L,对应的敏感度为58.8%,特异度为81.0%;NEFA的最佳分界值为0.47 mmol/L,对应的敏感度为58.8%,特异度为90.5%;产前7 d时,血清BHBA和NEFA可作为酮病的预警指标,BHBA的最佳分界值为0.34 mmol/L,对应的敏感度为76.5%,特异度为66.7%;NEFA的最佳分界值为0.32 mmol/L,对应的敏感度为94.1%,特异度为47.6%。本研究发现产前14和7 d血清ADP、LEP、抵抗素、IL-6和TNF-α均不适合为产后酮病的预警指标。然而,随预产期临近,该5种脂肪因子Logistic曲线的显著性逐渐向具有统计学意义接近,存在作为酮病预警指标的潜力。  相似文献   

硝化应激在神经型犬瘟热疾病发展中的临床意义     

朱道仙  刘莉  陆江  赵学刚  郝福星 《畜牧与兽医》2021,(2):110-114

为了研究硝化应激在神经型犬瘟热疾病发展中的作用,并探讨其对该病的诊断价值,选取犬瘟热临床病例113例,其中有神经症状组(NCD)共37例,无神经症状组(NNS)共76例,并设25例无犬瘟热健康犬作为对照组(CG)。就诊时分别采取血液和脑脊液,测定一氧化氮(NO)、神经元型一氧化氮合成酶(nNOS)和神经元特异性烯醇化酶(NSE)水平。结果显示:NCD组2种组织液中的NO、nNOS和NSE升高,显著高于NNS组(P<0.05)与CG组(P<0.01);ROC曲线分析发现,2种组织液中的NO、nNOS和NSE对神经型犬瘟热的诊断具有良好的灵敏度和特异性,血浆中以nNOS最佳,截断值为230.1 ng/mL,脑脊液中以NSE最佳,截断值为3.67 ng/mL;NSE对神经型犬瘟热具有一定预后价值,血浆中NSE<2.07的病例死亡率显著低于NSE≥2.07的病例。结果表明:神经型犬瘟热疾病发展中伴有硝化应激和神经损伤,血浆与脑脊液中的NO、nNOS和NSE具有对神经型犬瘟热的诊断及预测作用。  相似文献   

围产期奶牛氧化应激初步预警体系的建立     

孙光野  张翠羽  杨威  夏成  张洪友  徐闯 《中国兽医学报》2019,(3):529-534,540

旨在通过对围产期奶牛能量负平衡指标与其他血液生化指标的分析,初步建立围产期奶牛氧化应激的预警体系。本试验以能量负平衡为判定标准,BHBA>1.2 mmoI/L,NEFA>0.4 mmoI/L,GLU<3 mmoI/L为发病组,BHBA<1.2 mmoI/L,NEFA<0.4 mmoI/L,GLU>3 mmoI/L为健康对照组,各采集围产期发病组和对照组各25头奶牛的血液并对血中能量代谢、矿物质代谢、微量元素代谢、氧化应激、免疫抑制指标进行独立样本T检验和spearman相关分析,并利用logistic二元逻辑回归预测疾病,最后利用ROC分析确立分界值和诊断效果。结果显示:发病组奶牛血液中的Ca、GSH-Px、CAT、Ve、Mg、Se、Cu水平小于健康组;发病组奶牛血液中的IL-1、IL-6、CRP、IL-2、HP、TNF-α的水平高于健康组,表明能量负平衡的奶牛机体存在氧化应激并伴有炎症和矿物质代谢紊乱。奶牛血液中的Ca、Mg、GSH-Px、CAT、Cu、Se、Ve与BHBA呈显著的负相关,IL-1、IL-2、IL-6、HP、TNF-α与BHBA呈显著的正相关;奶牛血液中的Ca、Mg、GSH-Px、CAT、Ve、Cu与NEFA呈显著的负相关,IL-2、HP、TNF-α与NEFA呈显著的正相关;奶牛血液中的Ca、GSH-Px与GLU呈显著的正相关,IL-6、HP与GLU呈显著的负相关。经回归分析确定Ca、Mg、GSH-Px、CAT、Cu、Se、Ve、IL-1、IL-2、IL-6、TNF-α、HP、BHBA 13项指标与围产期泌乳奶牛氧化应激相关,经ROC分析确立GSH-Px、CAT、Se、IL-6、IL-2、TNF-α、BHBA 7项指标可作为氧化应激发生的预警指标。结果表明,因此,能量负平衡、VE、Se缺乏、炎性因子增多和矿物质代谢紊乱是奶牛产后氧化应激发生的风险因素,但VE和Ca未成为氧化应激的预警指标。初步证实了围产期奶牛氧化应激发生的预警指标,为今后更有效的监控和预防奠定理论基础。  相似文献   

基于ROC曲线的玛纳斯河灌区土壤盐分空间分析     

辛明亮  吕廷波  何新林  曹玉斌  王萌萌 《灌溉排水学报》2016,(12):45-50

选择我国干旱内陆河玛纳斯河流域平原灌区为研究对象,基于区域采样点数据,应用试验诊断评价方法——受试者工作特征曲线(ROC曲线)分析了不同采样时间各土层土壤含盐量的变化以及对多种空间插值方法进行了评价,最后选择估值效果较好的空间插值方法绘制了土壤盐分空间分布图,并与传统评价方法进行了对比。结果表明,基于ROC曲线,4月、10月表层土壤盐分变化剧烈,其变化敏感于其他土层,盐分存在表聚现象,ROC曲线下面积最大且贡献均为正;传统交叉检验和ROC曲线分析均得出利用经验贝叶斯克里格方法进行表层土壤含盐量空间插值效果最优;研究区表层土壤盐分具有明显的连续变化和存在局部聚集的现象;不同采样时间土壤盐分空间分布差异显著,总体表现为低值区位于南部山区和东北部,高值区主要分布在平原水库周围以及下游地势低处。ROC曲线能够很好地应用于土壤盐分空间分析领域。  相似文献   

假苍耳的地理分布及潜在适生区预测   总被引：1，自引：0，他引：1  

许志东  丁国华  刘保东  池春玉  肖玮  金晓霞  李春烨 《草业学报》2012,21(3):75-83

外来入侵植物假苍耳在中国东北部地区快速蔓延,威胁自然生态系统的结构并对人们的健康产生危害。本研究通过野外调查获得假苍耳在东北地区的分布范围,采用Maxent模型软件对假苍耳在我国及世界的潜在适生区进行预测,运用ArcGIS软件中的自然间断点分级法进行适生等级分类,ROC曲线评价Maxent模型预测的准确性,并结合刀切法及环境变量反应曲线判断分析影响假苍耳分布的主要环境变量。结果表明,假苍耳的适生区较广,扩散范围可能继续增大。我国的华北地区、东北地区、华东地区、华中地区、西南地区、西北地区均存在适生区,高度适生区主要分布在我国的黑龙江省、吉林省、辽宁省、内蒙古自治区、山西省、陕西省、河南省、河北省、山东省、北京市、天津市、新疆维吾尔族自治区。世界范围内除南极洲外均存在假苍耳的适生区,高度适生区主要在北美洲的加拿大和美国,非洲的摩洛哥,欧洲的西班牙、克罗地亚、匈牙利、斯洛伐克、波黑、塞尔维亚、马其顿、保加利亚、格鲁吉亚、亚美尼亚和阿塞拜疆,亚洲的土耳其、伊朗、哈萨克斯坦、吉尔吉斯斯坦、塔吉克斯坦、中国、朝鲜、韩国和日本。上述适生区预测显示出假苍耳适宜生长在年均温度较高,最冷月份最低温和最冷季度平均温度较低的中纬度地区。  相似文献   

岷江上游干旱河谷区灌丛植被土壤有机碳稳定性特征   总被引：1，自引：0，他引：1  

姜广争  宫渊波  陈林武  左琴  刘金鑫  岳艳杰  张兴华 《水土保持学报》2011,25(5):209-214

灌丛在岷江上游分布广泛,是干旱河谷区重要的物种,砍伐、开垦和过度放牧是导致其退化的根本驱动力。通过选取灌丛植被下土壤剖面,并以其被开垦后的农地（50a）及退耕荒坡（10a）为参照,对其土壤有机碳、重组有机碳及易氧化碳含量进行分析比较。结果表明：灌丛植被各层土壤总有机碳及重组有机碳均极显著大于对照的,对照间差异不显著,在0-100cm土壤剖面,对照1退耕荒坡及对照2农地土壤有机碳较灌丛植被的最高降幅分别达60.42%,70.92%,重组有机碳最高降幅则分别达76.54%,68.92%,并且灌丛及对照2农地土壤重组碳在0-100cm土层剖面上表现出先升高,然后再降低的分布特征,对照1退耕荒坡则在整个土壤剖面呈递减趋势;灌丛植被土壤易氧化碳均值高于对照的,与退耕荒坡的差异显著,但与农地的差异不显著,在0-100cm土层中,灌丛及对照土壤易氧化碳含量表现出先降低、升高、再降低的分布特征;相关分析表明,总有机碳与重组有机碳和易氧化碳达到极显著相关水平。综上表明灌丛植被保护土壤有机碳稳定能力极显著高于退耕荒坡和农地的,并且农地退耕也有利于土壤有机碳稳定性的提高。  相似文献   

Modelling the biological invasion of Prosopis juliflora using geostatistical-based bioclimatic variables under climate change in arid zones of southwestern Iran     

Mohadeseh AMIRI  Mosfata TARKESH  Mohammad SHAFIEZADEH 《干旱区科学》2022,14(2):203-224

Invasive species have been the focus of ecologists due to their undesired impacts on the environment.The extent and rapid increase in invasive plant species is recognized as a natural cause of global-biodiversity loss and degrading ecosystem services.Biological invasions can affect ecosystems across a wide spectrum of bioclimatic conditions.Understanding the impact of climate change on species invasion is crucial for sustainable biodiversity conservation.In this study,the possibility of mapping the distribution of invasive Prosopis juliflora(Swartz)DC.was shown using present background data in Khuzestan Province,Iran.After removing the spatial bias of background data by creating weighted sampling bias grids for the occurrence dataset,we applied six modelling algorithms(generalized additive model(GAM),classification tree analysis(CTA),random forest(RF),multivariate adaptive regression splines(MARS),maximum entropy(Max Ent)and ensemble model)to predict invasion distribution of the species under current and future climate conditions for both optimistic(RCP2.6)and pessimistic(RCP8.5)scenarios for the years 2050 and 2070,respectively.Predictor variables including weighted mean of CHELSA(climatologies at high resolution for the Earth’s land surface areas)-bioclimatic variables and geostatistical-based bioclimatic variables(1979–2020),physiographic variables extracted from shuttle radar topography mission(SRTM)and some human factors were used in modelling process.To avoid causing a biased selection of predictors or model coefficients,we resolved the spatial autocorrelation of presence points and multi-collinearity of the predictors.As in a conventional receiver operating characteristic(ROC),the area under curve(AUC)is calculated using presence and absence observations to measure the probability and the two error components are weighted equally.All models were evaluated using partial ROC at different thresholds and other statistical indices derived from confusion matrix.Sensitivity analysis showed that mean diurnal range(Bio2)and annual precipitation(Bio12)explained more than 50% of the changes in the invasion distribution and played a pivotal role in mapping habitat suitability of P.juliflora.At all thresholds,the ensemble model showed a significant difference in comparison with single model.However,Max Ent and RF outperformed the others models.Under climate change scenarios,it is predicted that suitable areas for this invasive species will increase in Khuzestan Province,and increasing climatically suitable areas for the species in future will facilitate its future distribution.These findings can support the conservation planning and management efforts in ecological engineering and be used in formulating preventive measures.  相似文献   

A Comparison of Multiple Allergen Simultaneous Tests Using Allergen-Specific IgE Concentration and Intradermal Skin Tests in Atopic Horses With Pollen Allergy     

《Journal of Equine Veterinary Science》2020

Intradermal tests (IDTs) and measurement of specific immunoglobulin E class (sIgE) levels in sera are the most common and reliable methods used in allergological clinical practice. The purpose of this study was to explore the sensitization of pollen allergy in atopic horses with pollinosis and to assess the diagnostic value of the multiple allergen simultaneous tests (MASTs) compared with that of the IDT. Twenty-one Malopolski horses with typical skin hypersensitivity symptoms during pollen seasons were enrolled. Intradermal tests were performed, and allergen-specific IgE concentrations in sera were measured using a monoclonal anti-IgE antibody to pollens of grass, weeds, trees, and cultivated plants. The highest sensitization rate was for Secale cereale (IDT, 76%; MAST, 66.7%), grass (IDT, 71%; MAST, 57%), and Brassica napus (IDT, 52.4%; MAST, 57%). Receiver operating characteristic (ROC) curve analysis and the area under the ROC curve (AUC) showed that Betula spp., Alnus spp., and Corylus spp. had the highest AUC at 0.854, followed by Secale cereale (AUC = 0.796), Plantago lanceolata (AUC = 0.726), Brassica napus (AUC = 0.704), and a grass allergen mixture (AUC = 0.695). The mean AUC for all allergens was 0.712 (0.604–0.867). The overall sensitivity of the sIgE plant horse panel was 78% (range, 68%–90%), the specificity was 86.3% (range, 64%–100%), and the accuracy was 79% (range, 64%–87%). Statistical kappa (κ) agreement between the MAST and IDT was reached for tree (κ = 0.767), rye (κ = 0.687), colza (κ = 0.671), and grass (κ = 0.664) pollen. The MAST showed a favorable agreement with the IDT and can be used for the detection of sIgE in atopic horses with pollinosis.  相似文献