玉米赤霉烯酮对动物繁殖性能的毒性作用及其机制     

宁春妹  安佳秀  赵颖  杨艺 《动物营养学报》2023,(4):2166-2174

玉米赤霉烯酮（ZEA）一种由镰刀菌属产生的次级代谢物，化学结构与内源性雌激素相似，因此，ZEA可通过与雌激素竞争性结合雌激素受体，导致动物机体生殖激素代谢紊乱，诱发生殖器官形变及功能障碍，严重危害动物繁殖系统，解决ZEA污染问题己经成为当务之急。本文对ZEA的理化特性、毒性作用等进行综述，以期为有效缓解ZEA毒性及提高动物繁殖性能、促进畜牧业健康发展提供理论依据。  相似文献   

獭兔玉米赤霉烯酮中毒的诊断     

刘吉山  肖跃强  唐世云  谢金文  姚春阳  莫玲  黄兵 《动物医学进展》2016,(5):129-132

山东某獭兔场饲养的3 000只母兔发生了一种以流产及青年兔死亡为特征的疾病,通过对发病兔进行细菌培养及兔病毒性出血症病毒的RT-PCR以及饲料中的玉米赤霉烯酮、呕吐毒素及黄曲霉毒素进行鉴别检测,首先排除细菌病及兔病毒性出血症发病的可能,并从饲料中检测3种毒素的含量分别为1 196.1、130.8、6μg/kg,经过对照饲料标准发现玉米赤霉烯酮严重超出标准(标准为300μg/kg)。通过饲喂试验发现这次发病与所饲喂的饲料有关,诊断为玉米赤霉烯酮中毒。  相似文献   

不同植物精油对玉米赤霉烯酮的脱毒效果研究     

周英焕 《现代农业科技》2021,23(23)

试验旨在研究植物精油对玉米赤霉烯酮（zearalenone, ZEA）的脱毒效果。选用肉桂精油、花梨木精油、棕榈精油、薄荷精油、橘子精油作为脱毒物质,分别添加植物精油于玉米赤霉烯酮标准品溶液中,采用酶联免疫法（ELISA）测定毒素含量。研究5种植物精油用量为85 μL/10 mL,48 h的脱毒效果;选择其中3种脱毒效果较好的植物精油,研究在24 h、48 h、72 h,用量为85 μL/10 mL时的脱毒效果;研究3种植物精油用量为75 μL/10 mL、85 μL/10 mL、95 μL/10 mL,48 h时的脱毒效果。结果表明,植物精油可以降低ZEA含量,且作用时间与用量不同,脱毒效果有差异。  相似文献   

玉米赤霉烯酮的生殖毒性研究进展   总被引：2，自引：0，他引：2  

王青  ;周丹朝  ;赵煜  ;周宏超  ;刘晓强  ;李引乾  ;杨鸣琦 《畜牧兽医杂志》2014,(4):32-35

玉米赤霉烯酮是一种特殊毒性的生物毒素,它的强雌激素样作用对动物机体的伤害很大,不仅引起动物流产、死胎、低产仔率等生殖机能异常,还可以导致繁殖功能下降、不育、畸形等.已有研究表明,ZEA对肝脏、肾脏、生殖系统和免疫系统产生明显的损伤,对细胞和遗传性也有毒性作用.目前,虽然国内外学者做了大量的研究,来揭示玉米赤霉烯酮损害机体生殖功能的机制,但关于它影响机体的生殖性能更详尽的机制还亟待进一步的研究.文章主要针对玉米赤霉烯酮的生殖毒性进行了综述,总结出ZEA对生殖系统的毒性机制,从而为临床预防及治疗繁殖疾病提供理论依据.  相似文献   

冬小麦越冬期茎尖超微结构变化与玉米赤霉烯酮的关系     

赵德刚  孟繁静 《中国农业大学学报》1997,2(3):15-20

应用酶联免疫技术和电镜技术，研究了冬小麦品种燕大１８１７越冬期内源玉米赤霉烯酮（ＺＥＮ）含量和茎尖超微结构的变化，结果表明，随着秋末冬初气温逐渐降低，日照缩短，茎尖ＺＥＮ含量逐渐增加，１１月下旬含量达到高峰，随后急剧下降，到１月下旬又出现一个小的含量高峰。在小麦茎尖ＺＥＮ含量出现高峰前后（１１月底，１２月初），茎尖细胞中线粒体和质体的体积增大，形状也发生变化，线粒体从低温诱导前的圆球形变为长形、哑  相似文献   

大麦茎尖中玉米赤霉烯酮动态及与发育之关系     

张富明  何立人 《仲恺农业技术学院学报》1994,7(1):1-6

用硅胶薄层层析扫描法检测了大麦品种安特１３（冬型）、Ｖ—２４（半冬型）、８５Ｇ６３（春型）由生长锥未伸长至雌雄蕊分化８个时期茎尖中玉米赤霉烯酮的含量．发现不同品种或不同处理玉米赤霉烯酮的动态有差异；不同品种各处理二棱初至三联期间皆有一玉米赤霉烯酮含量高峰，此阶段可能在发育上具有重要意义。  相似文献   

玉米赤霉烯酮的代谢、毒性及其预防措施   总被引：3，自引：0，他引：3  

姜淑贞  杨维仁  杨在宾 《动物营养学报》2011,23(2):196-202

玉米赤霉烯酮(ZEA)是由镰孢属真菌产生的类雌激素毒素.由于气候条件不同,食品和饲料中ZEA浓度差异很大(从几个mg/kg至几千个mg/kg).本文综述了ZEA的吸收、代谢和生物转化机制,ZEA的生殖毒性、细胞毒性、免疫毒性和遗传毒性,并且从预防土壤中镰孢菌及其相关霉菌的污染、收获和收获后真菌毒素的控制、污染颗粒的物理...  相似文献   

The Effect of Caffeic Acid on Zearalenone-induced Ovarian Granulosa Cell Apoptosis in Mice     

PEI Yaping  ZHAO Jin  SUN Na  SUN Panpan  SUN Yaogui  FAN Kuohai  YIN Wei  LI Hongquan 《畜牧兽医学报》1956,51(12):3068-3075

Ovarian granulosa cells provide a special microenvironment for follicle formation and maturation through interaction with oocytes and their own secretion. A variety of harmful stimuli can cause granulosa cell apoptosis and metabolic disorders, reduce the quality of oocytes and have a negative impact on embryo formation. Zearalenone (ZEA) is a common cause of ovarian granulosa cells injury in the livestock industry, which is produced by mycotoxins, and lack of effective treatment drug. Therefore, in the current study zearalenone was used to induce ovarian granulosa cell injury and to explore the protective effect of caffeic acid on zearalenone-induced ovarian granulosa cell apoptosis in mice. Mouse ovarian granulosa cells were isolated by mechanical method, and indirect immunofluorescence was used to identify the isolated cells. MTT assay was used to determine the effect of caffeic acid on the activity of normal mouse ovarian granulosa cells.After granulosa cells were co-treated with caffeic acid (200, 100 and 50 μg·mL^-1) and ZEA for 24 hours, and control and ZEA group were set up at the same time, cell morphology and adherence were observed under a microscope. MTT was also used to detect cell viability. Caspase-3 mRNA expression level was detected by qRT-PCR. Cleaved-caspase-3 and cleaved-PARP protein expre-ssion levels were determined by Western blot. The results showed that positive FSHR staining appeared in cell cytoplasm of the test group, which confirmed that the isolated cells were mouse ovarian granulosa cells. The cell viability was above 90% which showed that caffeic acid had no toxic effect on granulosa cells. Compared with control group, ZEA group had smaller cell size, poor adherence, increased cell gap, and significant reduction in cell viability (P<0.001). Furthermore, the relative expression of caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein level were significantly increased (P<0.001) compared with the control group. After caffeic acid treatment, cell gap was reduced, adherence was tight, cell viability was significantly increased (P<0.001). Caffeic acid significantly reduced zearalenone-induced increase in caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein expression level (P<0.001). This study indicated that caffeic acid can restore granulosa cell viability by inhibiting ZEA-induced apoptosis.  相似文献   

Effect on hepatonephric organs, serum metabolites and oxidative stress in post-weaning piglets fed purified zearalenone-contaminated diets with or without Calibrin-Z     

Jiang SZ  Yang ZB  Yang WR  Wang SJ  Wang Y  Broomhead J  Johnston SL  Chi F 《Journal of animal physiology and animal nutrition》2012,96(6):1147-1156

The objectives of this study were to investigate the toxicity of zearalenone (ZEA) on hepatonephric organs, serum metabolites and oxidative stress of piglets and to evaluate the efficacy of Calibrin‐Z (CAZ) in preventing ZEA‐induced adverse effects. The experiment was conducted for 22 days using 36 piglets weaned at 21 days of age (Landrace × Yorkshire × Duroc, 18 females and 18 males; 8.84 ± 0.21 kg average body weight). Piglets of each gender were randomly allocated to the following six dietary treatments: (i) Control (basal diet only); (ii) Control + 1 g/kg CAZ; (iii) Control + 1 mg/kg ZEA; (iv) Control + 1 mg/kg ZEA + 1 g/kg CAZ; (v) Control + 1 mg/kg ZEA + 2 g/kg CAZ; (vi) Control + 1 mg/kg ZEA + 4 g/kg CAZ. Piglets were housed and fed individually for the entire experimental period. Blood samples were taken, and piglets were killed at the end of the experiment to obtain organs for physiological assessment. Results showed that piglets fed the ZEA‐contaminated diet had increased (p < 0.05) activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma‐glutamyltransferase (GGT), creatine kinase and cholinesterase, concentrations of urea, and creatinine in serum, and malondialdehyde (MDA) in serum and liver. Pigs fed the ZEA‐only diet also showed reductions in serum (p < 0.05) globulin, triglycerides and high‐density lipoproteins (HDL), and reductions in total superoxide dismutase (TSOD) and glutathione peroxidase (GSHPx) activity in both serum and liver. Supplementation of CAZ at the dosages of 1–4 g/kg to the diet containing 1.05 mg/kg ZEA linearly increased (p < 0.05) concentrations of triglycerides and HDL in serum, activity of TSOD and GSHPx in serum and liver, but linearly reduced (p < 0.05) all tested serum enzymes and lowered (p < 0.05) the elevated concentrations of urea, and creatinine in serum, and MDA in serum and liver caused by dietary ZEA. Piglets fed the ZEA‐contaminated diet showed increased (p < 0.05) relative weight of liver and kidney compared with the control, whereas only numerical improvement on relative weight of liver and kidney was observed with simultaneous addition of CAZ at 4 g/kg diet and ZEA. However, feeding the diet with CAZ alone at 1 g/kg had no impact on any of the measured parameters when compared to the control. It is suggested that feeding ZEA at 1.05 mg/kg exerted a deleterious effect on piglets, which was totally or partly ameliorated by dietary supplementation of CAZ at concentrations between 1 and 4 g/kg diet.  相似文献   

郑州某粮库小麦中玉米赤霉烯酮含量的检测     

徐新慧  晏猛  符锋  贺秀媛  周华东  张君涛  李小佳  邓立新  王建华 《动物医学进展》2012,33(4):59-62

为了解动物饲料小麦中是否含有玉米赤霉烯酮毒素,应用竞争性酶免疫分析法快速定量测定43个小麦样品中玉米赤霉烯酮毒素的含量,并进行了分析。结果显示,在被检的43个小麦样品中,含有玉米赤霉烯酮的有5个(5/43),检出率占11.6%,玉米赤霉烯酮含量超过国家粮食卫生标准的有3个(3/43),超标率占7.0%,说明作为畜禽饲料原料的小麦,存在引发动物玉米赤霉烯酮毒素中毒的潜在危险。本法适宜于大批量小麦样品中玉米赤霉烯酮含量的快速测定,这对于选择品质优良的小麦作为饲料、避免玉米赤霉烯酮对畜禽生长危害有重要意义。  相似文献