| 运用Cytb 和12s rRNA 基因鉴别梅花鹿(Cervus nippon)和马鹿(Cervus elaphus)的研究 |
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| 引用本文： | 李波,白素英,徐艳春,张伟,马建章.运用Cytb 和12s rRNA 基因鉴别梅花鹿(Cervus nippon)和马鹿(Cervus elaphus)的研究[J].林业研究,2006,17(2):160-162. |
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| 作者姓名： | 李波 白素英 徐艳春 张伟 马建章 |
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| 作者单位： | 东北林业大学野生动物资源学院 哈尔滨150040 |
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| 摘 要： | 本研究介绍了运用细胞色素b基因和12s核糖体RNA基因部分序列的系统学和核甘酸距离分析来鉴别降解的梅花鹿和马鹿样品。采用PCR和直接测序技术获得了8份嫌疑样品402bp细胞色素b基因序列,并与来自GenBank数据库27份同源的细胞色素b基因序列进行比对。3份嫌疑样品与梅花鹿的核甘酸距离值相同(0.026±0.006),小于梅花鹿与东部马鹿间最小的核甘酸距离值(0.036)。并且梅花鹿和马鹿的系统学分析表明这些样品与梅花鹿聚为一枝,因此可以推测它们来源于梅花鹿。同样的方法得出另3份嫌疑样品来源于马鹿。该结果被387bp12s核糖体RNA基因序列的系统学和核甘酸距离分析进一步证实。该方法是有效的,花费的时间少,能帮助减少同类野生动物案件的发生。图2表1参13。
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| 关 键 词： | 梅花鹿(Cervus nippon) 马鹿(Cervus elaphus) 细胞色素b基因(Cytb) 12s核糖体RNA基因(12s rRNA) |
| 文章编号： | 1007-662X（2006）02-0160-03 |
| 收稿时间： | 2005-10-18 |
| 修稿时间： | 2005-12-13 |
Identification of sika deer and red deer using partial cytochrome b and 12s ribosomal RNA genes |
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| Bo Li,Su-ying Bai,Yan-chun Xu,Wei Zhang,Jian-zhang Ma.Identification of sika deer and red deer using partial cytochrome b and 12s ribosomal RNA genes[J].Journal of Forestry Research,2006,17(2):160-162. |
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| Authors: | Bo Li Su-ying Bai Yan-chun Xu Wei Zhang Jian-zhang Ma |
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| Institution: | (1) College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, PR China |
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| Abstract: | A study was conducted on the identifications of the degraded samples of sika deer (Cervus nippon) and red deer (Cervus elaphus) by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved
by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank
database. The values of three nucleotide distance between three suspected samples and sika deer were identical (0.26±0.006),
which was smaller than the smallest nucleotide distance between eastern red deer and sika deer (0.036). Furthermore, phylogenetic
analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences
were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further
confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming
and helpful to reduce the related cases with wildlife.
Biography: LI Bo (1977–), male, Ph.D. candidate and assistant Professor of State Forestry Administration Detecting Center of Wild Fauna
and Flora, Northeast Forestry University, Harbin 150040, P. R. China. |
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| Keywords: | Sika deer (Cervus nippon) Red deer (Cervus elaphus) Cytochrome b gene (Cytb) 12s ribosomal RNA gene (12s rRNA) |
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