| 许氏平鲉微卫星标记的开发及评价 |
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| 引用本文： | 贾超峰,马海涛,姜海滨,刘相全,薛蕊.许氏平鲉微卫星标记的开发及评价[J].上海海洋大学学报,2014,23(3):345-350. |
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| 作者姓名： | 贾超峰 马海涛 姜海滨 刘相全 薛蕊 |
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| 作者单位： | 上海海洋大学 水产与生命学院, 上海 201306;山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室, 山东 烟台 264006;上海海洋大学 水产与生命学院, 上海 201306;山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室, 山东 烟台 264006;上海海洋大学 水产与生命学院, 上海 201306;山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室, 山东 烟台 264006;上海海洋大学 水产与生命学院, 上海 201306;山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室, 山东 烟台 264006;上海海洋大学 水产与生命学院, 上海 201306;山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室, 山东 烟台 264006 |
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| 基金项目： | 现代农业产业技术体系项目（nycytx-50）；烟台市科技发展计划（2013ZH088） |
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| 摘 要： | 采用磁珠富集法筛选具多态性的许氏平鲉微卫星标记,并用一个野生群体对其多态性进行了评价。三引物PCR法筛选出58个阳性克隆进行了测序,共得到47个含微卫星核心的DNA序列,筛选出15对具有多态性的微卫星引物。所有位点上共得到66个等位基因片段,每个位点的观测等位基因数(A)为2~14个,平均等位基因数为4.4,观测杂合度(Ho)、期望杂合度(He)范围分别为0.062 5~0.968 8、0.061 5~0.929 1。每个位点的多态信息含量(PIC)显示,有6个位点表现出高度或中度多态。经Bonferroni校正后,HJ2-32、HJ4-27、HJ4-45和HJ4-47四个位点的等位基因频率显著偏离了Hardy-Weinberg平衡。连锁不平衡检测表明,各位点间无连锁不平衡现象。
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| 关 键 词： | 许氏平鲉 微卫星 磁珠富集 |
| 收稿时间： | 1/2/2014 12:00:00 AM |
| 修稿时间： | 3/4/2014 12:00:00 AM |
Isolation and characterization of microsatellite markers for black rockfish Sebastes schlegeli |
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| JIA Chao-feng,MA Hai-tao,JIANG Hai-bin,LIU Xiang-quan and XUE Rui.Isolation and characterization of microsatellite markers for black rockfish Sebastes schlegeli[J].Journal of Shanghai Ocean University,2014,23(3):345-350. |
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| Authors: | JIA Chao-feng MA Hai-tao JIANG Hai-bin LIU Xiang-quan and XUE Rui |
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| Institution: | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, Shandong, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, Shandong, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, Shandong, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, Shandong, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, Shandong, China |
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| Abstract: | Polymorphic microsatellite markers were developed from black rockfish(Sebastes schlegelii) with an enriched partial genomic library by magnetic beads and the polymorphisms of these loci were assessed in 32 individuals from a wild population. We screened 58 positive clones by three-primers PCR method and sequenced them, then 47 sequences were detected containing microsatellites, and 15 pairs of polymorphic microsatellite primers were obtained. A total of 66 alleles were detected, with the number of alleles ranging from 2 to 14 (averaging 4.4). The observed and expected heterozygosity values ranged from 0.062 5 to 0.968 8 and 0.061 5 to 0.929 1, respectively. The values of polymorphism information content indicated, six loci were highly or moderately polymorphic. HJ2-32,HJ4-27,HJ4-45 and HJ4-47 significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found. The result demonstrates these markers will facilitate relevant research in S. schlegelii. |
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| Keywords: | Sebastes schlegelii microsatellite enrichment by magnetic beads |
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