| 猪K88、K99、987P、F41埃希氏大肠杆菌高密度发酵培养技术的初步探讨 |
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| 引用本文： | 巫,月,生.猪K88、K99、987P、F41埃希氏大肠杆菌高密度发酵培养技术的初步探讨[J].中国兽药杂志,2011,45(5):8-10. |
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| 作者姓名： | 巫 月 生 |
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| 作者单位： | 广东永顺生物制药有限公司,广州,萝岗,511356 |
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| 摘 要： | 采用高密度发酵和普通深层通气两种方法培养含K88、K99、987P、F41菌毛抗原的四株猪埃希氏大肠杆菌,对其培养液进行活菌数、OD值、pH值、效价的测定。实验结果表明,运用高密度发酵方法培养,各菌活菌数可达4.1×1010~4.9×1010CFU/mL,效价为211~213;运用普通深层通气方法培养,各菌活菌数为0.51×1010~0.59×1010CFU/mL,效价为24~25,可选择高密度发酵方法替代普通深层通气方法培养,用于制备猪埃希氏大肠杆菌K88、K99、987P、F41四价菌毛提纯苗。
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| 关 键 词： | 猪埃希氏大肠杆菌 K88、K99、987P、F41 菌毛抗原 高密度发酵培养 |
| 收稿时间： | 2/22/2011 3:06:21 PM |
| 修稿时间： | 4/8/2011 12:23:27 AM |
Study on High Density Fermentation of Escherichia coli K88,K99,987P and F41 |
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| wu yue sheng.Study on High Density Fermentation of Escherichia coli K88,K99,987P and F41[J].Chinese Journal of Veterinary Drug,2011,45(5):8-10. |
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| Authors: | wu yue sheng |
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| Institution: | Guangdong Winsun bio-pharmaceutical co., ltd |
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| Abstract: | Culture Escherichia coli fimbiral antigen K88,K99,987P AND F41 by bioreactor and culture-bottles,and then measure their viable count（colony forming unit）,OD,pH and titer.The experimental results show that viable count reached 4.1×1010～4.9×1010 CFU/mL and titer reached 211～213 when bacteria were cultured by bioreactor.Viable count reached 0.51×1010～0.59×1010 CFU/mL and titer reached 24～25 when bacteria were cultured by culture-bottles.The results suggest that bioreactor can be used to culture Escherichia coli fimbirial antigen K88,K99,987P and F41. |
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| Keywords: | Escherichia coli K88 K99 987P F41 Fimbirial antigen High density fermentation |
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