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| 沟叶结缕草的组织培养和无性系的建立 | |
| 引用本文： | 李国平,杨鹭生,胡文英,黄群策.沟叶结缕草的组织培养和无性系的建立[J].林业科学研究,2005,18(5):541-545. |
| 作者姓名： | 李国平 杨鹭生 胡文英 黄群策 |
| 作者单位： | 1. 郑州大学离子束生物工程省重点实验室,河南,郑州,450052;莆田学院环境与生命科学系,福建,莆田,351100 2. 莆田学院环境与生命科学系,福建,莆田,351100 3. 郑州大学离子束生物工程省重点实验室,河南,郑州,450052 |
| 基金项目： | 国家“十五”科技攻关项目“离子束应用技术研究”(2001BA302B) |
| 摘 要： | 通过研究不同基本培养基及植物生长调节组合对沟叶结缕草丛生芽诱导、增殖和愈伤组织诱导、分化的影响,建立起沟叶结缕草试管无性系。以地下匍匐根状茎的顶芽为外植株,在MS 6-BA 2 mg.L-1 NAA 0.1 mg.L-1培养基上诱导丛生芽形成,以MS KT 3 mg.L-1 NAA 0.1 mg.L-1作为继代增殖培养基,建立起丛生芽苗→不定芽发生→丛生芽苗速生试管无性系;以丛生芽苗基部为外植体,以MS 2.4-D 4 mg.L-1作为愈伤组织诱导培养基,以MS基本培养基作为愈伤组织分化培养基,建立起丛生苗→愈伤组织→愈伤组织分化→丛生苗试管无性系。试管无性系室外移栽成活率达95%以上。沟叶结缕草试管无性系的建立为细胞工程育种和基因转化的研究奠定了基础。 |
| 关 键 词： | 沟叶结缕草 组织培养 无性系 愈伤组织 分化 |
| 文章编号： | 1001-1498（2005）05-0541-05 |
| 收稿时间： | 11 23 2004 12:00AM |
| 修稿时间： | 2004-11-23 |
Tissue Culture of Zoysia matrella and the Establishment of Asexual System |
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| LI Guo-ping,YANG Lu-sheng,HU Wen-ying and HUANG Qun-ce.Tissue Culture of Zoysia matrella and the Establishment of Asexual System[J].Forest Research,2005,18(5):541-545. | |
| Authors: | LI Guo-ping YANG Lu-sheng HU Wen-ying and HUANG Qun-ce |
| Institution: | Provincial Key Laboratory of Ion Beam Bio-engineering,Zhengzhou University,Zhengzhou 450052,Henan,China;Department of Environment and Life Science,Putian University,Putian 351100,Fujian,China;Department of Environment and Life Science,Putian University,Putian 351100,Fujian,China;Department of Environment and Life Science,Putian University,Putian 351100,Fujian,China;Provincial Key Laboratory of Ion Beam Bio-engineering,Zhengzhou University,Zhengzhou 450052,Henan,China |
| Abstract: | The paper dealt with the studies on the tissue culture and the establishment of asexual system of Zoysia matrella.Two kinds of asexual system were established successfully based on careful studies on the methods and major factors for induction,differentiation and propagation of tufted shoots and calli.One asexual system was maintained through tufted shoots regeneration,using top buds of rhizome of Zoysia matrella as explants,MS + 6-BA 2mg·L-1 + NAA 0.1 mg·L-1 as the effective medium for adventitious shoot induction and MS + KT 3mg·L-1 + NAA 0.1 mg·L-1 as the suitable subculture medium for adventitious shoot propagation.Another asexual system was maintained through callus induction and differentiation,using the base of tufted shoots as explants,MS + 2,4-D 4 mg·L-1 as the optimum medium for callus induction and MS basic medium as the effective medium for differentiation in callus.And the plantlets got acclimatized well,with the survival rate up to 95%.Establishment of asexual system laid a solid foundation for further studies on breeding by means of somaclonal variation and Agrobacterium-mediated genetic transformation. |
| Keywords: | Zoysia metrella tissue culture asexual system callus differentiation |
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