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The taxonomic characterisation of annual Beta germplasm in a genetic resources collection using RAPD markers
Authors:Y Shen  H J Newbury  B V Ford-Lloyd
Institution:(1) School of Biological Sciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, U.K.
Abstract:Summary Annual beets in the genus Beta section Beta represent an important genetic resource. Representative accessions of annual beets from a beet germplasm collection were analysed using RAPD to assess the patterns of variation and relationships among them. Using arbitrary primers, markers showing variation across accessions were identified. A dendrogram of similarity was produced using these molecular markers. All the accessions analysed were classified into three major groups corresponding to species or subspecies macrocarpa, adanensis and maritima. Macrocarpa was shown to be the most divergent group in this section. Using RAPD molecular markers, it was possible to ascribe an accession to one of three taxonomic groups and overcome much of the confusion encountered when morphological traits are used for identification. The group of maritima was found to be more polymorphic than either the group of macrocarpa or adanensis at both accession and subspecies levels.
Keywords:Beta  genetic resources  RAPD  annual beets  molecular marker  polymorphism
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