| 新生猪肌组织成纤维细胞的分离培养和鉴定 |
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| 引用本文： | 邢蕾,邓昌彦,郑嵘,熊琪,吴健,熊远著.新生猪肌组织成纤维细胞的分离培养和鉴定[J].黑龙江畜牧兽医,2008(2):18-20. |
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| 作者姓名： | 邢蕾 邓昌彦 郑嵘 熊琪 吴健 熊远著 |
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| 作者单位： | 华中农业大学农业动物遗传育种与繁殖教育部重点实验室,湖北武汉430070 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家科技支撑计划
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湖北省重大专项 |
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| 摘 要： | 研究建立了猪肌组织成纤维细胞体外培养的模型,采用组织块培养方法获得了新生猪肌组织成纤维细胞并进行体外培养.观察细胞形态,通过绘制细胞的生长曲线来研究肌组织成纤维细胞的生长规律,利用RT-PCR和免疫细胞化学染色对所获得的细胞进行鉴定,再用双荧光素酶检测系统来检测细胞的转染效率.通过形态学观察、RT-PCR和免疫细胞化学染色结果表明,已成功培养了猪肌组织成纤维细胞,纯度达90%以上.
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| 关 键 词： | 猪 肌组织成纤维细胞 分离 细胞培养 鉴定 |
| 文章编号： | 1004-7034（2008）02-0018-03 |
| 收稿时间： | 2007-04-20 |
| 修稿时间： | 2007年4月20日 |
Isolating culture and identification of muscle tissue fibroblasts of the Neonatal Pig |
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| XING Lei, DENG Chang -yan, ZHENG Rong, et al.Isolating culture and identification of muscle tissue fibroblasts of the Neonatal Pig[J].Heilongjiang Animal Science And veterinary Medicine,2008(2):18-20. |
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| Authors: | XING Lei DENG Chang -yan ZHENG Rong |
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| Abstract: | This research was done in order to establish an ideal cellular model of porcine muscle tissue fibroblasts in vitro culture. Porcine muscle tissue fibroblasts in vitro culture were obtained by tissue pieces. Morphological observation and growth curve were used to evaluate the growing trait of porcine musole tissue fibroblasts. The cells were identified with RT - PCR and cellular immunochemical stain. Efficiency of cellular transfection were detected by Dual - Luciferase Reporter Assay System. The purity of cells was more than 90% by the method of morphological observation and RT - PCR and cellular immunochemical stain. |
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| Keywords: | Pig muscle tissue fibroblasts isolation cellular culture identification |
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