| 高效液相色谱法测定金花颗粒中绿原酸的含量 |
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| 引用本文： | 朱昆,侯学谦,李慧萍,刘玉梅.高效液相色谱法测定金花颗粒中绿原酸的含量[J].特产研究,2009,31(1):57-58. |
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| 作者姓名： | 朱昆 侯学谦 李慧萍 刘玉梅 |
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| 作者单位： | 1. 吉林大学中日联谊医院,吉林,长春,130021
2. 吉林省油用总医院,吉林,松原,138000 |
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| 摘 要： | 目的建立用高效液相色谱法测定金花颗粒中绿原酸含量的方法。方法采用十八烷基键合硅胶色谱柱（250mm×4.6mm,5μm）,以乙腈-0.4%磷酸溶液（15︰85）为流动相,流速为1.00mL/min,检测波长为327nm。结果绿原酸的线性范围为0.04～0.37μg（r=0.9994）,平均回收率（n=5）为99.94%（RSD=1.35%）。结论本方法简单、迅速、可靠。
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| 关 键 词： | 高效液相色谱 金花颗粒 绿原酸 |
HPLC Determination of Chlorogenic Acid Content in Jinhua Granule |
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| ZHU Kun,HOU Xue-qian,LI Hui-ping,LIU Yu-mei.HPLC Determination of Chlorogenic Acid Content in Jinhua Granule[J].Special Wild Economic Animal and Plant Research,2009,31(1):57-58. |
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| Authors: | ZHU Kun HOU Xue-qian LI Hui-ping LIU Yu-mei |
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| Institution: | ZHU Kun; HOU Xue-qian; LI Hui-ping; LIU Yu-mei(1.China-Japan Union Hospital ofJilin University; Changchun 130021; China; 2.Jilin Oil Field General Hospital; Songyuan 138000; China); |
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| Abstract: | Objective To develop an HPLC method for determining chlorogenic acid content in Jinhua Granule.Methods Diamonsil C18 column（250mm×4.6mm,5μm）was used as the stationary phase,the mixture of acetonitrile-0.4% phosphoric acid（15：85） was served as mobile phase,and its flow rate was at 1.0 mL/min.The wavelength for detection was at 327nm.Results The linearity range was 0.04～0.37μg（r =0.999 4）.The average recovery was 99.94%（RSD =1.35%）.Conclusion The method is simple,fast and reliable. |
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| Keywords: | HPLC Jinhua granule Chlorogenic acid |
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