Characterization of a 54-kDa heat-shock-inducible protein of Pasteurella haemolytica |
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| Institution: | 1. Department of Clinical and Health Psychology, University of Florida, PO Box 100165, Gainesville, FL 32610, United States;2. NIMH Center for the Study of Emotion and Attention, University of Florida, Gainesville, FL 32611, United States;3. Center for Movement Disorders and Neurorestoration, University of Florida, United States |
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| Abstract: | Growth-condition-dependent antigens play a role in the virulence or protective capacity of many organisms. Enhanced production of an approximately 54-kDa protein was detected in heat-shocked cultures of Pasteurella haemolytica. The heat-shock-inducible protein cross-reacted with antibodies to 60-kDa heat-shock proteins of Mycobacterium tuberculosis, Chlamydia, and Escherichia coli GroEL. A probe containing the E. coli groEL operon hybridized with fragments of P. haemolytica chromosomal DNA on Southern blots. Immunoblots of the 54-kDa protein using serum from 20 calves that were challenged experimentally with P. haemolytica resulted in band densities that were significantly different between calves with high and low lesion scores. Results of the study suggest that the 54-kDa heat-shock protein may be a growth-condition-dependent immunogen that is one component of resistance to pneumonic pasteurellosis. |
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