Rapid and sensitive detection of Staphylococcus species in milk by ELISA based on monodisperse magnetic particles |
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| Institution: | 1. CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal;2. Department of Chemistry, University of Beira Interior, R. Marquês de Ávila e Bolama, 6200-001 Covilhã, Portugal;3. Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain;4. Analytical Spectroscopy and Sensors Group (GEAS), Institute of Environmental Sciences (IUCA), University of Zaragoza, c/Pedro Cerbuna 12, 50009, Zaragoza, Spain;1. Eastern Kentucky University, 4126A New Science Building, Richmond, KY 40475, USA;2. Center for Applied Energy Research, University of Kentucky, 2540 Research Park Drive, Lexington, KY 40511, USA |
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| Abstract: | Staphylococcus spp. are the single most important cause of mastitis in dairy cows. Aiming for a rapid and sensitive method for detection of staphylococci, a magnetic bead-based enzyme-linked immunosorbent assay (ELISA) employing MAb for the detection of staphylococci in milk was developed. Pretreatment of the sample with lysostaphin increased the sensitivity of the assay. The method is specific for most of staphylococci which cause mastitis in bovines, detects between 104–105 organisms per ml and takes three hours. Thus, the principle for staphylococci ELISA based on monodisperse magnetic beads is a way for rapid detection of staphylococci in milk and possibly also for detecting staphylococci by direct testing of other clinical specimens. The system may be automated. |
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