| 奶牛乳房炎大肠杆菌分离株主要免疫原性蛋白纯化 |
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| 引用本文： | 辛凤艳,侯喜林,朴范泽.奶牛乳房炎大肠杆菌分离株主要免疫原性蛋白纯化[J].黑龙江八一农垦大学学报,2007,19(4):63-66. |
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| 作者姓名： | 辛凤艳 侯喜林 朴范泽 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,大庆,163319 |
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| 基金项目： | 国家重大科技攻关专项课题 |
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| 摘 要： | 应用奶牛乳房炎大肠杆菌分离株灭活菌苗免疫健康家兔,制备兔抗大肠杆菌高免血清。对该大肠杆菌菌体裂解产物进行SDS-PAGE并进行Westernblot分析,结果该菌株与稀释一定倍数的兔高免血清反应,产生3条特异性带,其中约33ku蛋白为主要免疫原性蛋白。对其进行电洗脱纯化,得到单一条带的目的蛋白。
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| 关 键 词： | 大肠杆菌 外膜蛋白 纯化 |
| 文章编号： | 1002-2090（2007）04-0063-04 |
| 收稿时间： | 2005-05-29 |
| 修稿时间： | 2005年5月29日 |
Purification of the Main Immunogenic Protein of Escherichia coli Isolated from Dairy Cows Suffering from Mastitis |
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| XIN Feng-yan,HOU Xi-lin,PIAO Fan-ze.Purification of the Main Immunogenic Protein of Escherichia coli Isolated from Dairy Cows Suffering from Mastitis[J].Journal of Heilongjiang August First Land Reclamation University,2007,19(4):63-66. |
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| Authors: | XIN Feng-yan HOU Xi-lin PIAO Fan-ze |
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| Abstract: | In order to prepare antiserum against Escherichia coli of rabbits, healthy rabbits were immunized by antiserum against Escherichia coli isolated from dairy cows suffering from mastitis. Lysate of the isolated E.coli was analyzed with SDS-PAGE and Western blot. The results showed that the lysate could react with diluted antiserum to produce three bands and the band of main immunogenic protein was at 33 ku. The main immunogenic protein could be purified to show a single band by electroneluting. |
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| Keywords: | Escherichia coli outer membrane protein purification |
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