| 洞庭湖水系鳜遗传结构 |
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| 引用本文： | 袁希平,李成,李鸿,王崇瑞,洪波.洞庭湖水系鳜遗传结构[J].淡水渔业,2012(5):8-12. |
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| 作者姓名： | 袁希平 李成 李鸿 王崇瑞 洪波 |
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| 作者单位： | 湖南省水产科学研究所;农业部长江中上游渔业资源环境科学观测实验站,中国水产科学院长江水产研究所 |
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| 基金项目： | 农业部长江中上游渔业资源环境重点野外科学观测实验站开放课题(YWTZ/1003) |
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| 摘 要： | 测定了采自洞庭湖水系6个地理位置(湘江常宁、沅江洪江、沅江桃源、澧水石门、洞庭湖万子湖和南岳坡)共100尾鳜(Siniperca chuatsi)的线粒体Cyt b基因DNA序列。结果显示:1 140 bp的Cyt b基因共检测到55个变异位点,100个样本得到9个单倍型。单倍型多样性指数(Hd)和核苷酸多样性指数(Pi)分别为0.777±0.033和0.019 70±0.000 74。洞庭湖的万子湖和南岳坡2个群体遗传多样性指数最高,湘江常宁群体遗传多样性指数最低。分子变异分析(AMOVA)结果显示:FST=0.098 03(P>0.05),9.8%的变异来自群体间,91.2%的变异来自群体内。除常宁群体分化显著外,其它群体没有出现明显遗传分化(两两之间FST在-0.065～0.024之间)。
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| 关 键 词： | 鳜(Siniperca chuatsi) 遗传结构 保留Cytb基因 洞庭湖 |
Population genetic structure of Mandarin fish(Siniperca chuatsi) in Dongting Lake |
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| YUAN Xi-ping,LI Cheng,LI Hong,WANG Chong-rui,HONG Bo.Population genetic structure of Mandarin fish(Siniperca chuatsi) in Dongting Lake[J].Freshwater Fisheries,2012(5):8-12. |
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| Authors: | YUAN Xi-ping LI Cheng LI Hong WANG Chong-rui HONG Bo |
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| Institution: | 1(1.Hunan Fisheries Institute,Changsha 410153,China;2.Scientific Observing and Experimental Station of Fishery Resources and Environment in the Upper and Middle Reaches of the Yangtze River,Ministry of Agriculture, Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China) |
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| Abstract: | Siniperca chuatsi,an endemic species to China,and its germplasm protection and resources utilization becomes more and more concerned.To know its genetic diversity and differentiation,the mitochondrial DNA Cyt b gene was amplified and sequenced for 100 individuals from six regions of Dongting Lake basin,including Changning,Hongjiang,Taoyuan,Shimen,Wanzihu and Nanyuepo.Altogether 55 variable nucleotide sites existed among the aligned sequences of 1140bp,and 9 haplotypes were found within 100 individuals.The average nucleotide diversity(Pi) was 0.019 7±0.000 74,while haplotype diversity(Hd) was 0.777±0.033.Genetic diversity was the highest in Wanzihu and Nanyuepo population,while that was the lowest in Changning population.AMOVA analysis demonstrated that FST was 0.098 03(P>0.05),small variances occurred among populations(9.8%) and most variances occurred within populations(91.2%).Except Changning population which had significant genetic differentiation,the other populations had no obvious differentiation(Fst among them were between-0.065 to 0.024). |
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| Keywords: | Siniperca chuatsi genetic structure Cyt b gene Dongting Lake |
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