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| 鲫肠道上皮细胞原代培养方法的研究 | |
| 引用本文： | 宋增福,吴天星,潘晓东.鲫肠道上皮细胞原代培养方法的研究[J].淡水渔业,2008,38(1):67-69,34. |
| 作者姓名： | 宋增福 吴天星 潘晓东 |
| 作者单位： | 1. 浙江大学动物科学学院,杭州,310029;上海水产大学生命学院,上海,200090 2. 浙江大学理学院,杭州,310029 3. 浙江大学动物科学学院,杭州,310029 |
| 基金项目： | 浙江省重大科技攻关课题，上海市高校优秀青年教师后备人选科研项目，上海水产大学校科研和校改项目 |
| 摘 要： | 探讨了健康鲫(Carassius auratus)幼鱼肠道上皮细胞的原代培养方法。结果显示,用含有抗生素、胶原蛋白酶Ⅰ、EDTA和胶原蛋白酶Ⅳ组成的D-Hanks液消化无菌分离的鲫肠道可以获得大量细胞团及绒毛隐窝;用含有抗生素、胎牛血清(FBS)、表皮生长因子(EGF)、胰岛素的DMEM培养液进行培养,并根据肠道上皮细胞与成纤维细胞贴壁时间的差异进一步纯化,连续培养12d后可以得到纯化的肠道上皮细胞。 |
| 关 键 词： | 鲫(Carassius auratus) 肠道上皮细胞 原代培养 |
| 文章编号： | 1000-6907-（2008）01-0067-03 |
| 收稿时间： | 2007-04-20 |
| 修稿时间： | 2007年4月20日 |
Study Primary Culture Methods of Intestinal Epithelial Cells of Crucian Carp(Carassius auratus) |
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| SONG Zeng-fu,WU Tian-xing,PAN Xiao-dong.Study Primary Culture Methods of Intestinal Epithelial Cells of Crucian Carp(Carassius auratus)[J].Freshwater Fisheries,2008,38(1):67-69,34. | |
| Authors: | SONG Zeng-fu WU Tian-xing PAN Xiao-dong |
| Abstract: | Methods of primary culture of intestinal epithelial cells of crucian carp were investigated by referring to that of human and with some modification.The results indicated that germ-freely separated intestine section of crucian carp digested by D-Hanks culture with compounded antibiotics,collagen enzymeⅠ,collagen enzyme Ⅳand EDTA could archive mass intestinal epithelial cells(IECs)and villus recesses.When the cells were cultured with DMEM culture containing compounded antibiotic,epithelial grow factor(EFG),insulin and fetal bovine serum(FBS)and purified according to adherence time between fibroid cells and IECs,the purified IEC could be obtained after 12-day continuing culture. |
| Keywords: | crucian carp(Carassius auratus) intestinal epithelial cell primary culture |
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