| 猪尿中糖皮质激素类药物残留的UPLC-MS/MS检测方法的研究 |
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| 引用本文： | 班付国,贾振民,吴宁鹏,宋志超.猪尿中糖皮质激素类药物残留的UPLC-MS/MS检测方法的研究[J].中国农业科技导报,2008,10(Z2):22-26. |
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| 作者姓名： | 班付国 贾振民 吴宁鹏 宋志超 |
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| 作者单位： | 河南省饲料产品质量监督检验站 |
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| 摘 要： | 建立猪尿中9种糖皮质激素类药物残留的UPLC-MS/MS检测方法。样品在酸性条件下酶解后,乙酸乙酯提取,再用HLB固相萃取柱和氨基固相萃取柱净化后,用液相色谱-串联质谱测定。9种药物在2~100ng/mL浓度范围内呈线性相关,在空白猪尿中添加1~5 ng/mL浓度范围内,平均回收率为55.0%~106.2%,批内变异系数为3.0%~19.1%,批间为1.3%~15.4%,检测限为0.5 ng/mL,定量限为1.0 ng/mL。本方法具有较好的灵敏度、准确度和精密度,能满足猪尿中此类药物残留的检测。
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| 关 键 词： | 猪尿 糖皮质激素 UPLC-MS/MS |
Determination of Glucocorticoids Rresidues in Pig Urine by UPLC-MS/MS |
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| BAN Fu-guo,JIA Zhen-min,WU Ning-peng,SONG Zhi-chao.Determination of Glucocorticoids Rresidues in Pig Urine by UPLC-MS/MS[J].Journal of Agricultural Science and Technology,2008,10(Z2):22-26. |
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| Authors: | BAN Fu-guo JIA Zhen-min WU Ning-peng SONG Zhi-chao |
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| Institution: | Henan Feed Quality Supervision and Testing Station|Zhengzhou 450008|China |
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| Abstract: | A method for determination of glucocorticoids residues in pig urine by UPLC-MS/MS was developed.The samples were deconjugated with β-glucuronidase/ arylsulfatase enzyme,and extracted by ethyl acetate,followed by clean-up with a dual HLB and aminopropyl cartridge.The analytes were quantified by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry(UPLC-MS/MS) operating in the negative ion mode.The assay for 9 glucocorticoids was linear over the range of 2~100 ng/mL with a h... |
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| Keywords: | UPLC-MS/MS |
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