| 多年生黑麦草愈伤组织遗传转化受体系统的建立 |
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| 引用本文： | 陈智勇,易自力,蒋建雄,覃静萍,刘清波,蔡能,唐小艳,孙鏖.多年生黑麦草愈伤组织遗传转化受体系统的建立[J].草原与草坪,2007(3):28-32. |
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| 作者姓名： | 陈智勇 易自力 蒋建雄 覃静萍 刘清波 蔡能 唐小艳 孙鏖 |
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| 作者单位： | 湖南农业大学,生物科学技术学院,湖南,长沙,410128 |
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| 基金项目： | 湖南省自然科学基金
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湖南省教育厅科研基金 |
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| 摘 要： | 多年生黑麦草成熟胚为外植体,选择CC+2,4-D 4 mg/L为诱导培养基,NB+6-BA 2 mg/L为分化培养基,1/2 NB+IAA 0.5 mg/L+NAA 0.5 mg/L为生根培养基,建立了多年生黑麦草胚性愈伤组织高频诱导与再生系统,确定了G418(150~200 mg/L)、Hpt(50 mg/L)、Basta(20~30 mg/L)的筛选临界浓度,确定了最佳分化时间,探讨了建立多年生黑麦草转化受体系统的必要条件。
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| 关 键 词： | 多年生黑麦草 愈伤组织 转化受体系统 |
| 文章编号： | 1009-5500（2007）03-0028-05 |
| 收稿时间： | 2006-12-04 |
| 修稿时间： | 2006-12-042007-04-18 |
Establishment of the transformation receptor system for Lolium perenne |
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| CHEN Zhi-yong,YI Zi-li,JIANG Jian-xiong,QIN Jing-ping,LIU Qing-bo,CAI Neng,TANG Xiao-yan,SUN Ao.Establishment of the transformation receptor system for Lolium perenne[J].Grassland and Turf,2007(3):28-32. |
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| Authors: | CHEN Zhi-yong YI Zi-li JIANG Jian-xiong QIN Jing-ping LIU Qing-bo CAI Neng TANG Xiao-yan SUN Ao |
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| Institution: | College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: | An efficient regeneration and transformation system for Lolium perenne by using mature seeds of three species was established.An efficient induction and regeneration system from embryonic calli of these species was setup and the conentrations of different antibiotics for callus growth was determined.The essensial requirement of establishing the transformation receptor system for Lolium perenne was discussed in terms of choosing plant tissues,confirming the effect medias and selecting the best regeneration time. |
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| Keywords: | Lolium perenne cullus transformation receptor system |
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