| 白草花叶病毒承德玉米分离物3′-cDNA 片段序列分析 |
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| 引用本文： | 崔小雯,高波,许斐斐,李向东,张春庆,苗洪芹.白草花叶病毒承德玉米分离物3′-cDNA 片段序列分析[J].植物病理学报,2012,42(1):32-36. |
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| 作者姓名： | 崔小雯 高波 许斐斐 李向东 张春庆 苗洪芹 |
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| 作者单位： | 1 山东农业大学植物保护学院植物病理学系, 泰安271018; 2 山东农业大学农学院, 泰安271018; 3 河北省农科院植保所, 保定071000 |
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| 基金项目： | 转基因生物新品种培育重大专项(2009ZX08003-014B);山东省科技攻关项目(2009GG10009021);山东省现代农业产业技术体系 |
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| 摘 要： | 采自河北承德11 个表现矮花叶症状的玉米样品,用甘蔗花叶病毒(Sugarcane mosaic virus, SCMV)和白草花叶病毒
(Pennisetum mosaic virus, PenMV)简并引物扩增了基因组3′ 端约2. 1 kb 的片段并进行测序。Blast 结果表明其中8 个样
品含有PenMV。扩增到的PenMV 序列均为2 135 nt,包括部分NIb 基因(985 nt)、完整的CP 基因(909 nt)和3′-UTR(241
nt)。这8 个分离物CP 基因和3′-UTR 与GenBank 上其他PenMV 分离物相应序列的核苷酸一致率分别为89. 8% ~ 93． 4%
和95. 9% ~ 97. 9% 。根据扩增的2 135 nt 序列和CP 基因序列构建系统发育树,8 个分离物与GenBank 上其他PenMV 分离
物都分为2 个组:山西组和承德组。重组分析表明CD9 的CP 基因存在重组。
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| 关 键 词： | 白草花叶病毒 玉米 系统发育分析 重组 |
| 收稿时间： | 2011-06-11; |
Sequencing and analysis of the 3′-terminal genome of Pennisetum mosaic virus isolates from maize plants in Chengde |
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| CUI Xiao-wen
,
GAO Bo
,
XU Fei-fei
,
LI Xiang-dong
,
ZHANG Chun-qing
,
MIAO Hong-qin.Sequencing and analysis of the 3′-terminal genome of Pennisetum mosaic virus isolates from maize plants in Chengde[J].Acta Phytopathologica Sinica,2012,42(1):32-36. |
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| Authors: | CUI Xiao-wen GAO Bo XU Fei-fei LI Xiang-dong ZHANG Chun-qing MIAO Hong-qin |
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| Institution: | 1 Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, China; 2 College of Agronomy, Shandong Agricultural University, Tai’an 271018, China; 3 Institute of Plant Protection, Hebei Academy of Agricultural Sciences, Baoding 071000, China |
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| Abstract: | Totally 11 maize samples showing dwarf mosaic symptoms were collected from Chengde,Hebei Province.The 3′-terminal 2.1 kb genomic fragments were amplified with degenerate primers for Sugarcane mosaic virus(SCMV) and Pennisetum mosaic virus(PenMV) and then sequenced.The Blast results showed that eight samples were infected with PenMV.The cloned genome of these eight isolates were all 2 135 nucleo-tides(nt) long,including partial NIb gene(985 nt),complete CP gene(909 nt) and the 3′-UTR(241 nt).The CP gene and 3′-UTR shared nt identities of 89.8%-93.4% and 95.9%-97.9%,respectively,with the corresponding PenMV sequences available in the GenBank.In the phylogenetic trees constructed with the 3′-terminal 2 135 nt sequence and CP gene,all the PenMV isolates were divided into two groups: Shanxi group(SX) and Chengde group(CD).Recombination event was detected in the CP gene of isolate CD9. |
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| Keywords: | Pennisetum mosaic virus maize phylogenetic analysis recombination |
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