| 患病草鱼肠道病原菌的分离鉴定及毒力研究 |
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| 引用本文： | 邹升,龚亮,李东杰,曹丽娜,李艳平,何昊城,丁学知,易敢峰,夏立秋.患病草鱼肠道病原菌的分离鉴定及毒力研究[J].水产科学,2019(2):152-162. |
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| 作者姓名： | 邹升 龚亮 李东杰 曹丽娜 李艳平 何昊城 丁学知 易敢峰 夏立秋 |
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| 作者单位： | 湖南师范大学生命科学学院淡水鱼类发育生物学国家重点实验室微生物分子生物学湖南省重点实验室;大北农水产科技集团 |
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| 基金项目： | 国家自然科学基金资助项目(31770106);国家"973"基础研究计划项目(2012CB722301);湖南省重大专项(2017NK1030);湖南省生物发育工程及新产品研发协同创新中心项目(20134486) |
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| 摘 要： | 自环洞庭湖区精养鱼池患病草鱼肠道中分离获得4种病原菌,进行16S rRNA基因和gyrB基因序列测定和在线比对,并对草鱼进行回归感染。随后进行4种病原菌的药敏特征、溶血活性试验,并利用PCR方法检测了6种毒力因子的携带情况。试验结果表明,病原菌分别为温和气单胞菌、异常嗜糖气单胞菌、类志贺邻单胞菌和格氏乳球菌;4种病原菌回归感染草鱼,均能使草鱼患病致死,自死亡的草鱼体内均能分离得到相应感染的病原菌;4种病原菌对多种抗生素具有耐药性,且在脱纤维绵羊血血平板上检测到了温和气单胞菌、异常嗜糖气单胞菌AaB007的溶血活性;PCR结果表明,4种病原菌携带多种毒力因子。研究结果可为环洞庭湖区草鱼精养鱼池健康养殖及病害防治提供科学依据。
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| 关 键 词： | 草鱼 病原菌 16S RRNA基因 gyrB基因 回归感染 毒力基因 |
Isolation,Identification and Virulence of Pathogenic Bacteria in Gut of Diseased Grass Carp Ctenopharyngodon idellus |
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| ZOU Sheng,GONG Liang,LI Dongjie,CAO Lina,LI Yanping,HE Haocheng,DING Xuezhi,YI Ganfeng,XIA Liqiu.Isolation,Identification and Virulence of Pathogenic Bacteria in Gut of Diseased Grass Carp Ctenopharyngodon idellus[J].Fisheries Science,2019(2):152-162. |
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| Authors: | ZOU Sheng GONG Liang LI Dongjie CAO Lina LI Yanping HE Haocheng DING Xuezhi YI Ganfeng XIA Liqiu |
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| Institution: | (State Key Laboratory of Development Biology of Freshwater Fishes,Hunan Provincial Key Laboratory of Microbial Molecular Biology,College of Life Science,Hunan Normal University,Changsha 410081,China;Da Bei Nong Aquatic Science and Technology Group,Zhaoan 363500,China) |
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| Abstract: | Four pathogenic strains were isolated from intestine of diseased grass carp ( Ctenopharyngodon idellus ) collected from an intensive fish farming ponds in Dongting Lake area. The 16S rRNA gene sequencing,gyrB gene sequencing and BLAST analysis revealed that these four pathogenic bacterial strains were Aeromonas sobria , A. allosaccharophila , Plesiomonas shigelloide and Lactococcus garvieae . The infection tests indicated that all these four pathogenic strains were lethal to grass carp,and the corresponding pathogenic bacteria were isolated from the dead grass carp. Subsequently,we studied the drug sensitivity and hemolytic activity of these four strains,and it was approved that they carried the six toxicity genes by PCR. The results showed that the four pathogenic strains had resistance to a variety of antibiotics and many toxicity genes. The findings provide important scientific basis for healthy aquaculture of grass carp in intensive fish culture ponds in Dongting Lake area and that more attention should be paid to prevention and control of freshwater fish diseases. |
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| Keywords: | grass carp pathogenic strain 16S rRNA gene gyrB regression infection test toxicity gene |
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