| 光肩星天牛纤维素酶的性质研究 |
| |
| 引用本文： | 陈敏,卢孟柱,张志毅.光肩星天牛纤维素酶的性质研究[J].中国林学,2002,4(2):43-47. |
| |
| 作者姓名： | 陈敏 卢孟柱 张志毅 |
| |
| 作者单位： | 1. 北京林业大学生物科学与技术学院,北京,100083
2. 中国林业科学研究院林业研究所,北京,100091 |
| |
| 摘 要： | 该文对光肩星天牛 (Anoplophoraglabripennis)幼虫纤维素酶的特性进行了研究 .内切 β 1,4 葡聚糖酶 (内切葡聚糖酶 ,Cx)和β 1,4 葡萄糖苷酶 (β 葡萄糖苷酶 )的最适作用温度均为 4 0℃ ,最适作用pH值分别为 4 4 5 6和 4 8,内切葡聚糖酶具有较广泛的pH值和温度作用范围 ,在 2 5 5 0℃之间能保持80 %以上活性 ,pH 3 2 7 2之间能保持 6 0 %以上的酶活性 .内切葡聚糖酶的热稳定性也稍强于 β 葡萄糖苷酶 ,但在 6 0℃温育 30min后 ,二者均丧失活性 .用含 0 1%CMC的聚丙烯酰胺凝胶电泳方法检测到光肩星天牛的内切葡聚糖酶具有两种同工酶 ,从非变性聚丙烯酰胺凝胶中回收该两条酶带 ,并在SDS 聚丙烯酰胺凝胶电泳中呈单一酶带 ,分子量分别为 2 6kD和 39kD .纯化的同工酶处于进一步研究中
|
| 关 键 词： | 光肩星天牛 纤维素酶的特性 内切葡聚糖酶 β葡萄糖苷酶 同工酶 |
Characteristics of Cellulases from Anoplophora glabripennis Motsch ( Coleoptera: Cerambycidae) |
| |
| Chen Min,LU Mengzhu,Zhang Zhiyi.Characteristics of Cellulases from Anoplophora glabripennis Motsch ( Coleoptera: Cerambycidae)[J].Forestry Studies in China,2002,4(2):43-47. |
| |
| Authors: | Chen Min LU Mengzhu Zhang Zhiyi |
| |
| Abstract: | The property of major cellulases from the guts of Anoplophora glabripennis larvae have been char acterized. The optimal temperatures of both β-l,4-glucosidase (β-glucosidase) and endo-β-1,4-endoglucanase (endoglucanase, Cx) are 40℃. The β-glucosidase was optimally active at pH 4.8, while the optimal activity of the endoglucanase occurred at pH 4.4-5.6. The endoglucanase was active with a wide range of pH and temperature, the levels of activity from 25℃ to 50℃ were more than 80%, and the activity remained 60% between pH 3.2 and pH 7.2. The endoglucanase exhibited higher thermal stability than β-glucosidase. Both enzymes lose their activities by heat treatment at 60℃. Two isozymes of endoglucanase were detected in sodium carboxymethylcellulose polyacrymide gels (CMC-gel) by chemical colorization, and purified by elution from the gel slices. The molecular weights of the two isozymes were estimated as 26kD and 39kD respectively. Moreover molecular characteristics of the two isozymes are currently underway. |
| |
| Keywords: | Anoplophora glabripennis characteristics of cellulases endoglucanase isozymes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
