| 濒危植物巴东木莲RAPD扩增反应体系的建立 |
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| 引用本文： | 汤子春,李雪萍,陈发菊,乐超银,姚伟,梁宏伟,戴建武,陈凡,何正权.濒危植物巴东木莲RAPD扩增反应体系的建立[J].福建林业科技,2006,33(1):20-23. |
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| 作者姓名： | 汤子春 李雪萍 陈发菊 乐超银 姚伟 梁宏伟 戴建武 陈凡 何正权 |
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| 作者单位： | 1. 三峡大学生物技术研究中心,湖北,宜昌,443002;三峡大学天然产物研究与利用湖北省重点实验室,湖北,宜昌,443002
2. 三峡大学生物技术研究中心,湖北,宜昌,443002;北京林业大学生物科学与技术学院,北京,100083 |
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| 基金项目： | 三峡大学重大科研项目(611202)资助 |
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| 摘 要： | 以巴东木莲叶片为材料,通过对反应体系中Mg2+、dNTP、Taq酶、模板、引物浓度等影响因素的优化,确定了一套适于巴东木莲RAPD分析的稳定反应体系,即25μl的总反应体系中:模板DNA 100 ng,Mg2+2.1 mM,引物0.7μM,dNTP0.20 mM,TaqDNA聚合酶3 U。
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| 关 键 词： | 巴东木莲 RAPD 优化 扩增反应 濒危植物 |
| 文章编号： | 1002-7351（2006）01-0020-04 |
| 收稿时间： | 2005-08-10 |
| 修稿时间： | 2005-08-10 |
Optimization for RAPD Reaction of Manglietia patungensis Hu |
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| TANG Zi-chun,LI Xue-ping,CHEN Fa-ju,YUE Chao-yin,YAO Wei,LIANG Hong-wei,DAI Jian-wu,CHEN Fan,HE Zheng-quan.Optimization for RAPD Reaction of Manglietia patungensis Hu[J].Journal of Fujian Forestry Science and Technology,2006,33(1):20-23. |
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| Authors: | TANG Zi-chun LI Xue-ping CHEN Fa-ju YUE Chao-yin YAO Wei LIANG Hong-wei DAI Jian-wu CHEN Fan HE Zheng-quan |
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| Institution: | 1. Biotechnology Research Center, China Three Gorges University, Yichang 443002, China ; 2. Key Lab for Natural Product Research and Utilization, China Three Gorges University, Yichang 443002, China ; 3. School of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China |
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| Abstract: | RAPD technology was first employed to study the rare and endangered plant Manglietia patungensis Hu in this paper.An efficient and stable RAPD reaction system for the plant was established through the optimization of the reaction factors:the concentration of Mg~(2+),dNTP,Taq DNA polymerase,template and the primers.The results showed that the optimized RAPD reaction conditions were 100 ng template DNA,2.1 mM Mg~(2+),0.7 μ M primer,0.20 mM dNTP and 3 unit Taq DNA polymerase in a total of 25μl reaction mixture. |
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| Keywords: | Mangliettia patungnesis Hu RAPD optimization reaction dangerous plant |
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