Standardization of large scale production of homologous live attenuated PPR vaccine in India |
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| Authors: | Raveendra Hegde Amitha R Gomes S M Byregowda Paramananda Hugar P Giridhar C Renukaprasad |
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| Institution: | (1) Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore, 560 024, Karnataka, India;(2) Veterinary Officer, Veterinary Hospital, Doddaballapur, Bangalore, Karnataka, India |
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| Abstract: | Live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the Indian
Veterinary Research Institute, Muktheswar, India. Vero cells between 130–150 passages with six percent foetal calf serum were
used for the production of vaccine. The cells were infected with 0.01 multiplicity of infection and harvested when the cytopathic
effect was 80%. The vaccine was freezedried in order to maintain the stability of the vaccine. Identity test and titration
was performed and the vaccine titre was monitored to be minimum of 105/100 doses. In-house sterility tests and quality control tests using experimental animals and small ruminants were performed.
The vacuum and moisture content of the vaccine were also regulated to be within the normal limits. |
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| Keywords: | Peste des petits ruminants Homologous vaccine Live attenuated India Vaccine production Vero cells TCID50 Quality control tests Multiplicity of infection |
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