| 马归液对腺性膀胱炎大鼠膀胱组织Bcl-2、Bax表达的影响 |
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| 作者姓名： | 蔡蔚 谢海平 龙蠡 谢晓 杨华伟 |
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| 作者单位： | 湖南中医药大学第一附属医院, 湖南 长沙 410007,湖南中医药大学第一附属医院, 湖南 长沙 410007,湖南中医药大学第一附属医院, 湖南 长沙 410007,湖南中医药大学第一附属医院, 湖南 长沙 410007,湖南中医药大学第一附属医院, 湖南 长沙 410007 |
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| 基金项目： | 湖南省自然科学基金项目（13JJ5029）；湖南省教育厅优秀青年项目（15B175）。 |
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| 摘 要： | 目的 通过观察马归液对腺性膀胱炎大鼠模型膀胱组织的影响，为运用马归液治疗腺性膀胱炎提供实验基础和理论依据。方法 首先通过对大鼠膀胱灌注大肠埃希菌成功建立腺性膀胱炎大鼠模型，然后对造模成功的大鼠分别灌注马归液第1组为1次/周；马归液第2组为2次/周、吡柔比星、生理盐水8周，再对各组大鼠膀胱组织进行病理学检查、透射电镜检测和Bcl-2、Bax基因表达检测，以观察各组大鼠膀胱黏膜组织结构的变化。结果 经马归液灌注，马归液1组、马归液2组病理学检查显示膀胱组织正常无炎性病变；Bax、Bcl-2灰度均值比较，马归液2组、马归液1组的灰度均值与生理盐水组比较，均有统计学意义（P<0.05），马归液2组与马归液1组比较，无统计学意义（P>0.05）；Bcl-2/Bax中，马归液1组、马归液2组分别与吡柔比星组、生理盐水组组间比较中均有统计学意义（P<0.05），马归液2组与马归液1组比较无统计学意义（P>0.05）。结论 马归液对大鼠腺性膀胱炎膀胱黏膜有明显治疗效果，具有修复作用，且一周灌注两次疗效最佳。
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| 关 键 词： | 腺性膀胱炎 大鼠 马归液 Bcl-2 Bax |
| 收稿时间： | 2015/10/16 0:00:00 |
Effect of Magui Liquid on the Expression of Bcl-2 and Bax in Bladder Tissue of Rats with Cystitis Glandularis |
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| Authors: | CAI Wei XIE Haiping LONG Li XIE Xiao and YANG Huawei |
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| Institution: | The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China and The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China |
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| Abstract: | Objective To observe the effect of Magui liquid on bladder tissues in rats with cystitis glandularis, and to provide the experimental basis and theoretical basis for the treatment of cystitis glandularis. Methods Firstly, the model rats with cystitis glandularis were successfully established by bladder instillation of Escherichia coli. The successful model rats were given Magui liquid (group 1:once a week; group 2:twice a week), pirarubicin and physiological saline for eight weeks. Then, the bladder tissues of rats in each group were given pathological examination and electron microscopy assay, and the Bcl-2, Bax expressions were detected. The changes of the structure of bladder mucosa in rats were detected. Results After the perfusion of Magui liquid, the pathological examination of Magui liquild groups showed that the bladder tissue had no inflammatory disease. Compared with the physiological saline group, the Bax and Bcl-2 gray mean were statistically significant (P<0.05), the diffrences between Magui liquild groups were not statistically significant (P>0.05). Compared with pirarubicin and physiological saline group, the Bcl-2/Bax in Magui liquild groups was statistically significant (P<0.05), the Bcl-2/Bax between Magui liquid groups has no statistically significant (P>0.05). Conclusion Magui liquild shows obvious effect and repair functioon on the glandular cystitis bladder mucosa of rats, and the rats with best curative effect are given perfusion for two times a week. |
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| Keywords: | cystitis glandularis rats Magui liquild Bcl-2 Bax |
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