| 山羊源多动物链球菌的分离与鉴定 |
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| 引用本文： | 董文龙,王巍,耿昕颖,勾长龙,盛宇轩,王雪,马红霞,高云航.山羊源多动物链球菌的分离与鉴定[J].上海交通大学学报(农业科学版),2016(5):23-26, 40. |
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| 作者姓名： | 董文龙 王巍 耿昕颖 勾长龙 盛宇轩 王雪 马红霞 高云航 |
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| 作者单位： | 吉林农业大学 动物科学技术学院,长春130118,吉林农业大学 动物科学技术学院,长春130118,吉林农业大学 动物科学技术学院,长春130118,吉林农业大学 动物科学技术学院,长春130118,吉林农业大学 动物科学技术学院,长春130118,吉林省农业技术推广总站,长春130033,吉林农业大学 动物科学技术学院,长春130118,吉林农业大学 动物科学技术学院,长春130118 |
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| 基金项目： | 吉林省现代农业产业技术体系建设专项(2015-24)；吉林省动物疾病防治研究创新团队(20111820) |
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| 摘 要： | 从患病山羊鼻腔内分离到1株优势菌,该菌株为革兰氏阳性球菌,成对排列或为链状结构,能够发酵麦芽糖、乳糖、蔗糖和甘露醇并能产生碱性磷酸酶。对16S rDNA基因进行测序和Blast分析结果显示其与已知多动物链球菌16S rDNA序列(Y18026.1)的同源性为99%。动物回归致病性试验表明,该分离株对山羊具有较强致病性并命名为SP-1。药敏试验结果显示,该菌对氯霉素、阿米卡星、恩诺沙星、环丙沙星敏感;对阿奇霉素、红霉素、氟苯尼考、庆大霉素、阿莫西林、磺胺间甲氧嘧啶耐药。
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| 关 键 词： | 山羊 多动物链球菌 分离鉴定 |
| 收稿时间： | 2015/9/24 0:00:00 |
Isolation and Identification of Streptococcus pluranimalium from Goat |
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| DONG Wen-long,WANG Wei,GENG Xin-ying,GOU Chang-long,SHENG Yu-xuan,WANG Xue,MA Hong-xia and GAO Yun-hang.Isolation and Identification of Streptococcus pluranimalium from Goat[J].Journal of Shanghai Jiaotong University (Agricultural Science),2016(5):23-26, 40. |
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| Authors: | DONG Wen-long WANG Wei GENG Xin-ying GOU Chang-long SHENG Yu-xuan WANG Xue MA Hong-xia and GAO Yun-hang |
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| Institution: | College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China and College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China |
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| Abstract: | A dominant bacterium was isolated from nasal cavity of sick goat.It was named SP-1 as this paired or chained Gram-positive coccus was highly pathogenic to goat and its 16S rDNA has 99% sequence homology with that of known Streptococcus pluranimalium (Y18026.1).The bacterium can ferment maltose,lactose,sucrose and mannitol and generate alkaline phosphatase.It was sensitive to chloramphenicol,amikacin,enrofloxacin and ciprofloxacin but resistant to azithromycin,erythromycin,florfenicol,gentamicin,amoxicillin and sulfamonomethoxine. |
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| Keywords: | goat Streptococcus pluranimalium isolation and identification |
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