| 茶皂素对茶尺蠖毒性作用初步研究 |
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| 引用本文： | 雷攀登,孙钦玉,张家侠,袁争,王文杰.茶皂素对茶尺蠖毒性作用初步研究[J].农学学报,2016,6(3):16-20. |
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| 作者姓名： | 雷攀登 孙钦玉 张家侠 袁争 王文杰 |
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| 作者单位： | 安徽省农业科学院茶叶研究所,安徽省农业科学院茶叶研究所,安徽省农业科学院茶叶研究所,安徽省农业科学院茶叶研究所,安徽省农业科学院茶叶研究所 |
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| 基金项目： | 安徽省农科院院长青年创新基金“茶皂素对茶尺蠖毒性作用机理研究”(13B0733)，“60Co-γ辐照茶尺蠖的不育虫细胞分裂与染色体结构变异的研究”(14B0842)；国家现代农业产业技术体系建设专项(CARS-23)。 |
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| 摘 要： | 为研究茶皂素的生物活性及对昆虫作用机理，测定了茶皂素对茶尺蠖卵及幼虫的毒性作用。结果表明：施用浓度为0.1、0.2、0.4、0.8、1、2、4、8 mg/mL茶皂素后，卵孵化率分别为21.9%、16.2%、18.2%、11.7%、7.7%、6.2%、5.4%、5.9%，随着茶皂素浓度的提高，杀卵作用增强，高浓度茶皂素(?1 mg/mL)对茶尺蠖卵具有较强的杀卵作用。1、2、4、8 mg/mL茶皂素溶液处理茶尺蠖3 龄幼虫并饲养5 天后，无幼虫死亡，说明茶皂素对茶尺蠖3 龄幼虫没有明显的触杀作用，对茶尺蠖的直接毒杀活性较弱。不同浓度的茶
皂素对茶尺蠖3 龄幼虫均有一定的拒食作用，随着浓度的增加，拒食效果增强，4、8 mg/mL 的茶皂素拒食作用较强，分别达到80.0%、85.7%。取食过茶皂素的3 龄、4 龄、5 龄幼虫虫体长度和重量与未取食的没有明显差异，饲喂8 mg/mL及以下浓度茶皂素的茶尺蠖生长发育均未受到明显影响。
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| 关 键 词： | 外源硒 外源硒 叶用莴苣 氨基酸组分 有机酸组分 风味品质 |
| 收稿时间： | 2015/9/27 0:00:00 |
| 修稿时间： | 2015/10/24 0:00:00 |
Toxicity Effect of Tea Saponin on Ectropis oblique |
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| Abstract: | To investigate the bioactive and the insecticidal mechanism of tea saponin on pest, toxicity effect of tea saponin on eggs and larvae of Ectropis oblique was determined. Toxicity test for eggs of E. oblique was conducted at the concentration of 0.1, 0.2, 0.4, 0.8, 1, 2, 4, 8 mg/mL, respectively, and the corresponding hatch rate was 21.9%, 16.2%, 18.2%, 11.7%, 7.7%, 6.2%, 5.4%, 5.9%, respectively. This result indicated that toxicity effect on eggs of E. oblique increased with the increase of tea saponin concentration. Leaves were dipped in tea saponin solution at the concentration of 1, 2, 4, 8 mg/mL, respectively, and then fed the third instar larvae of E. oblique. The results showed that all the larvae survived, meaning that tea saponin had no significant toxicity on the third instar larvae of E. oblique. Antifeeding effect of tea saponin on the third instar larvae was also determined. The results indicated that tea saponin had antifeeding effect on the third instar larvae and the effect increased with the increase of the concentration. When the concentration of tea saponin was at 4, 8 mg/mL, the antifeeding rate was 80.0%, 85.7%, respectively. Body length and weight of the third, fourth and fifth instar larvae which were treated by tea saponin had no significant difference compared with that of the control group, indicating that feeding tea saponin at or below the concentration of 8 mg/mL had no significant effect on the growth and development of E. oblique. |
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| Keywords: | Tea Saponin Ectropis oblique Antifeeding Effect |
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