| 生物素化DNA与链霉亲和素绑定初步研究 |
| |
| 引用本文： | 杨秋萍,何珊珊,王志民.生物素化DNA与链霉亲和素绑定初步研究[J].上海交通大学学报(农业科学版),2016(6):9-13, 20. |
| |
| 作者姓名： | 杨秋萍 何珊珊 王志民 |
| |
| 作者单位： | 上海交通大学 农业与生物学院,上海200240,上海交通大学 农业与生物学院,上海200240,上海交通大学 农业与生物学院,上海200240 |
| |
| 基金项目： | 上海交通大学创新基金支持项目(X198775) |
| |
| 摘 要： | 将生物素标记的DNA与链霉亲和素(streptavidin,STV)按4∶1摩尔比连接,用原子力显微镜(atomic force microscope,AFM)对混合物表征,观察到所有可能的几种DNA-STV复合物。并按照不同摩尔浓度比和孵育时间配制DNA与STV混合液,探讨了影响结合效率的因素,初步得到用生物素-链霉亲和素系统批量制备1STV-1DNA单分子复合物的最佳制备条件,为未来单分子DNA的大规模快速制备奠定了基础。
|
| 关 键 词： | 生物素-链霉亲和素系统 DNA-STV复合物 AFM 1STV-1DNA单分子复合物 |
| 收稿时间： | 2016/3/17 0:00:00 |
Preliminary Studies on the Binding of Biotinylated DNA to Streptavidin |
| |
| YANG Qiu-ping,HE Shan-shan and WANG Zhi-min.Preliminary Studies on the Binding of Biotinylated DNA to Streptavidin[J].Journal of Shanghai Jiaotong University (Agricultural Science),2016(6):9-13, 20. |
| |
| Authors: | YANG Qiu-ping HE Shan-shan and WANG Zhi-min |
| |
| Institution: | School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China and School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China |
| |
| Abstract: | Mixture conjugation products between biotinylated DNA and streptavidin(STV)at a molar ratio of 4:1 were characterized using atomic force microscope(AFM),and a few but not all types of DNA-STV conjugates were observed.Meanwhile,conjugation efficiency was investigated under various DNA to STV ratios and incubation time.The optimum parameters to produce 1STV-1DNA conjugate were preliminary obtained,providing a foundation for facile and large-scale preparation of single molecule DNA conjugate. |
| |
| Keywords: | biotin-streptavidin system DNA-STV conjugation AFM single 1STV-1DNA conjugate |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《上海交通大学学报(农业科学版)》浏览原始摘要信息 |
| 点击此处可从《上海交通大学学报(农业科学版)》下载免费的PDF全文 |
|
