| 耐盐性Imt1基因表达载体的构建及其在酵母中的高效表达 |
| |
| 引用本文： | 董云洲,沈波.耐盐性Imt1基因表达载体的构建及其在酵母中的高效表达[J].中国农业科学,1997,30(4):50-55. |
| |
| 作者姓名： | 董云洲 沈波 |
| |
| 作者单位： | 中国农业科学院生物技术研究中心，北京 100081 美国亚利桑那大学生物化学系 比利时Leuven大学分子细胞生物学系 |
| |
| 基金项目： | 农业部1995～1996年度访问学者资助 |
| |
| 摘 要： | 将Imt1（Inositol O-methyltransferase，肌醇甲基转移酶）构建成酵母表达载体pBD#-3，在酵母双突变体gpd#+（-）(glycerol 3-phosphate-dehydrogenase,3-磷酸甘油脱氢酶)中高效表达，使gpd#+（-）突变株对氯化钠的耐性由2.5%提高到5.0%。生化分析表明，在每mg干酵母细胞内积累高达2.2nmol的Ononitol(芒柄醇)含量。Western blot分析证明了Imt1基因的表达。这一研究为植物耐盐基因工程开辟了一条新途径。
|
| 关 键 词： | Imt1基因 耐盐性 酵母 高效表达 |
Construction and Highly Effective Expression of Imt1 Gene in Yeast Cells |
| |
| Dong Yunzhou,Shen Bo,Stefan Hohmann,Hans Bohnert.Construction and Highly Effective Expression of Imt1 Gene in Yeast Cells[J].Scientia Agricultura Sinica,1997,30(4):50-55. |
| |
| Authors: | Dong Yunzhou Shen Bo Stefan Hohmann Hans Bohnert |
| |
| Institution: | Dong Yunzhou 1 Shen Bo 2 Stefan Hohmann 3 Hans Bohnert 2 |
| |
| Abstract: | Baker's yeast (Saccharomyces cerevisiae) accumulates under osmotic stresses, such as salt stress, glycerol to intracellular concentrations exceeding 1 mol/L and can then survive 10% NaCl. This is accomplished by induction of enzymes encoded by two genes, gpd 1 and gpd 2, and cessation of glycerol export to the medium. A double mutant with deletions of both gpd genes can only survive on 2.5% NaCl. We have supplied this mutant with Imt1 gene that lead to the production of D ononitol (Imt1 from Mesembryanthemum crystallium). The Imt1 gene leads to the accumulation of D ononitol in yeast to a concentration of 2.2 nmol/mg dry cells and the cells can survive on up to 5% NaCl. Western blotting showed the expression of the Imt1 gene in yeast cells. This experiment result demonstrated that the induced production of polyols is one of the main mechanisms with which some plants are tolerant to salt stress. This provide a new pathway for genetic engineering of salt sensitive crops. |
| |
| Keywords: | Imt1 gene Salt tolerance Double mutant yeast Highly effective expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
