Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland |
| |
| Authors: | H Olczak-Woltman A Masny G Bartoszewski A P&#;ucienniczak K Niemirowicz-Szczytt |
| |
| Institution: | Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, ul. Nowoursynowska 159, 02-776 Warszawa;and;Institute of Biotechnology and Antibiotics, ul. Staro?cińska 5, 02-516 Warszawa, Poland |
| |
| Abstract: | Several strains of Pseudomonas syringae pathovar (pv.) lachrymans and related bacterial pathogens were isolated from cucumber ( Cucumis sativus ) leaves collected in central and southern Poland in 2001 and 2002. Twenty five original strains, together with five reference strains of P. syringae pv. lachrymans , pv. syringae and pv. tomato , were genetically characterized by PCR-RFLP (polymerase chain reaction ? restriction fragment length polymorphism), ADSRRS (amplification of DNA fragments surrounding rare restriction sites), and PCR-MP (PCR ? melting profiles) fingerprinting techniques. Genetic similarity analyses of the PCR-RFLP and ADSRRS fingerprints showed that strains of P. syringae pv. lachrymans form distinct clusters. The results also indicated that the ADSRRS and the PCR-MP fingerprinting techniques may serve as more efficient tools for evaluating genetic similarity among pathovars and strains of P. syringae than PCR-RFLP. The 25 strains showed diverse pathogenicity to cucumber seedlings and biochemical tests were varied. The syrB gene was identified in four cucumber strains, characterized as P. syringae pv. syringae . |
| |
| Keywords: | ADSRRS angular leaf spot Cucurbitaceae molecular characterization PCR-melting profiles PCR-RFLP |
|
|
