| 尾叶桉优良无性系组培快繁研究 |
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| 引用本文： | 廖焕琴,黄晓玲,潘文,张卫华.尾叶桉优良无性系组培快繁研究[J].广东林业科技,2017,33(2):36-41. |
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| 作者姓名： | 廖焕琴 黄晓玲 潘文 张卫华 |
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| 作者单位： | 1. 广东省林业科学研究院 / 广东省森林培育与保护利用重点实验室,广东 广州,510520;2. 仲恺农业工程学院,广东 广州,510225 |
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| 基金项目： | 广东省省级科技计划项目“广东主要商品林种业关键技术研究”（2015B070701009）。 |
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| 摘 要： | 文章以尾叶桉(Eucalypt urophylla)优良新无性系的无菌组培苗为材料,MS培养基为基本培养基,研究6-BA、NAA不同质量浓度配比对尾叶桉无菌幼苗丛芽增殖率,以及生根粉种类、质量浓度、不同IBA和NAA配比对不同尾叶桉无性系生根率的影响.试验结果表明,当6-BA质量浓度为0.01 mg/L,丛芽增殖率在NAA质量浓度为0.01、0.1 mg/L时递增;而NAA质量浓度为1、5 mg/L时,则有利于生根;筛选出适宜尾叶桉优良无性系ZQUA10的增殖培养基为MS+0.01 mg/L 6-BA+0.01 mg/L NAA+30 g/L蔗糖+6 g/L卡拉胶,增殖系数达到4.7;筛选出适宜尾叶桉优良无性系ZQUA13的生根培养基为1/2MS+0.5 mg/L ABT1+30 g/L蔗糖+6 g/L卡拉胶,生根率达到57.5%;筛选出适宜尾叶桉优良无性系ZQUB58的生根培养基为1/2MS+1 mg/L IBA+0.5 mg/L NAA+30 g/L蔗糖+6 g/L卡拉胶,生根率达到100%.
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| 关 键 词： | 尾叶桉 组织培养 再生体系 |
| 收稿时间： | 2017/2/23 0:00:00 |
| 修稿时间： | 2017/3/2 0:00:00 |
The Research of Eucalypt urophylla on Tissue Culture and Rapid Propagation |
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| Liao Huanqin,Huang Xiaoling,Pan Wen and ZHANG Weihua.The Research of Eucalypt urophylla on Tissue Culture and Rapid Propagation[J].Forestry Science and Technology of Guangdong Province,2017,33(2):36-41. |
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| Authors: | Liao Huanqin Huang Xiaoling Pan Wen and ZHANG Weihua |
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| Institution: | Guangdong Academy of Forestry,Zhongkai University of Agriculture and Engineering,Guangdong Academy of Forestry,Guangdong Academy of Forestry |
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| Abstract: | The paper studied the effects of different 6-BA and NAA concentrations on aseptic seedling clus-ter buds proliferation rate as well as the effects of different rooting powder and concentration on rooting rate of Eucalyptus urophylla tissue culture seedling with MS medium as basic medium. The study aimed to provid the-oretical guidance for the establishment of efficient tissue culture and rapid propagation system and accelerat the efficient utilization of fine varieties and factory production of E. urophylla. The results showed: With the treatment 0.01 mg/L 6-BA and 0.01、0.1 mg/L NAA, the cluster buds proliferation rate reached the highest; it was benefi-cial to the growing of roots when NAA concentration was 1、5 mg/L; the proper medium for clonal variety of E. urophylla(ZQUA10 ) was MS + 0.01 mg/L 6-BA +0.01 mg/L NAA + 30 g/L sucrose +6 g/L carrageenan, the pro-liferation coefficient reached 4.7. The proper medium for clonal variety of E. urophylla (ZQUA13) was 1/2MS +0.5 mg/L ABT1 + 30 g/L sucrose +6 g/L carrageenan, the rooting rate reached 57.5%. The proper medium for clonal variety of E. urophylla (ZQUB58) was 1/2MS+1 mg/L IBA+0.5 mg/L NAA+ 30 g/L sucrose +6 g/L carra-geenan and the rooting rate reached 100%. |
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| Keywords: | Eucalyptus urophylla tissue culture regeneration system |
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