| 茶树休眠芽与萌动芽抑制消减杂交文库的构建与初步分析 |
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| 引用本文： | 王新超,杨亚军,陈亮,马春雷,姚明哲.茶树休眠芽与萌动芽抑制消减杂交文库的构建与初步分析[J].茶叶科学,2010,30(2):129-135. |
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| 作者姓名： | 王新超 杨亚军 陈亮 马春雷 姚明哲 |
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| 作者单位： | 1. 中国农业科学院茶叶研究所茶树资源和改良研究中心/国家茶树改良中心,浙江 杭州 310008; 2. 中国农业科学院研究生院,北京 100081 |
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| 基金项目： | 国家自然科学基金项目(30872059);;现代农业(茶叶)产业技术体系项目 |
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| 摘 要： | 为了解茶树越冬芽休眠及其解除的分子机理,以休眠芽和萌动芽为材料,构建了茶树休眠芽与萌发芽的正、反向抑制消减杂交文库,并从两个文库各随机挑选96个阳性克隆进行序列测定和生物信息学分析。建库质量分析结果表明,所构建的正、反向消减文库有效富集了差异基因,消减效率达到要求,插入片段集中于250~750 bp之间,文库质量较好。对正、反向文库随机挑选的阳性克隆测序和分析结果表明,正、反向文库在基因表达谱上存在一定的差异,萌动芽文库在亚细胞器形成、转运载体等方面的基因与休眠芽文库有较大差异,而休眠芽文库在抗氧化、翻译调控以及胁迫响应等方面的基因与萌动芽文库有较大差异。这两个文库的建立为进一步了解茶树芽休眠与解除的分子机制奠定了基础。
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| 关 键 词： | 茶树(Camellia sinensis) 芽休眠 表达序列标签 抑制消减杂交 |
| 收稿时间： | 2009-11-04 |
Construction and Preliminary Analysis of the Suppression Subtractive Hybridization cDNA Libraries between Dormant and Sprouting Buds of Tea Plant(Camellia sinensis) |
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| WANG Xin-chao,YANG Ya-jun,CHEN Liang,MA Chun-lei,YAO Ming-zhe.Construction and Preliminary Analysis of the Suppression Subtractive Hybridization cDNA Libraries between Dormant and Sprouting Buds of Tea Plant(Camellia sinensis)[J].Journal of Tea Science,2010,30(2):129-135. |
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| Authors: | WANG Xin-chao YANG Ya-jun CHEN Liang MA Chun-lei YAO Ming-zhe |
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| Institution: | 1. Research Center for Tea Germplasm and Improvement, Tea Research Institute of Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China; 2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Bud dormancy and its release are important biological phenomena in tea plant (Camellia sinensis). In order to understand the molecular mechanism of tea bud dormancy and its release, 2 SSH-cDNA libraries were constructed using dormant and sprouting buds. The forward library contained genes preferentially expressed in sprouting buds and the reverse library contained genes preferentially expressed in dormant buds. One hundred and ninety two positive clones were randomly selected for sequencing and bioinformati... |
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| Keywords: | tea plant (Camellia sinensis) bud dormancy expressed sequence tag (EST) suppression subtractive hybridization (SSH) |
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