| 卤虫幼体高密度短期营养强化条件的探究 |
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| 引用本文： | 高美荣,邓洪刚,邢明威,加晶,周广航,龙菲平,吴星宇,王谝,隋丽英.卤虫幼体高密度短期营养强化条件的探究[J].上海海洋大学学报,2024,33(2). |
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| 作者姓名： | 高美荣 邓洪刚 邢明威 加晶 周广航 龙菲平 吴星宇 王谝 隋丽英 |
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| 作者单位： | Tianjin University of Science and Technology,Tianjin University of Science and Technology,Tianjin University of Science and Technology,国投生物科技投资有限公司；微藻生物能源与资源北京市重点实验室,国投生物科技投资有限公司；微藻生物能源与资源北京市重点实验室,国投生物科技投资有限公司；微藻生物能源与资源北京市重点实验室,国投生物科技投资有限公司；微藻生物能源与资源北京市重点实验室,国投生物科技投资有限公司；微藻生物能源与资源北京市重点实验室,天津科技大学 海洋与环境学院 亚洲区域卤虫参考中心 |
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| 基金项目： | 水生动物免疫增强剂的研发及其在水产养殖中的应用 |
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| 摘 要： | 短期卤虫营养强化是提高水产生物饵料卤虫幼体营养价值的重要途径。本研究利用商业化生产的三角褐指藻(Phaeodactylum tricornutum)、湛江等边金藻(Isochrysis zhanjianggensis)、破囊壶菌(Schizochytrium aggregatum)干粉对初孵卤虫幼体进行强化,探索最佳卤虫强化条件,并在最适条件下分别强化美国大盐湖和乌兹别克斯坦咸海卤虫幼体,探究两种单胞藻和破囊壶菌对不同品系卤虫n-3高度不饱和脂肪酸(n-3 HUFA)的强化效果。结果表明,利用湛江等边金藻,在不同强化剂量(0.05,0.1,0.15和0.2 g/L)和不同强化时间(12 h, 18 h和24 h)条件下进行高密度卤虫强化(150,200和250 ind./mL),强化后卤虫存活率均在85~90%,且随着强化时间的延长和强化剂量的增加,卤虫个体体长和总生物量有所增加。考虑到育苗场对卤虫个体大小的要求和可操作性,建议卤虫幼体最佳强化密度为150~200 ind./mL,微藻强化剂量为0.1~0.2 g/L,强化时间为12~24 h。卤虫n-3 HUFA强化效果与强化剂种类和卤虫品系密切相关。对于大盐湖卤虫而言,强化三角褐指藻和湛江等边金藻强化的卤虫幼体EPA含量分别为7.39 mg/g dw和3.20 mg/g dw,与初孵卤虫EPA含量1.63 mg/g dw相比显著增加；对于咸海卤虫幼体,与初孵卤虫EPA含量14.35 mg/g dw相比,两种单胞藻强化的卤虫幼体EPA有所增加(分别为17.59 mg/g dw和17.26 mg/g DW),这与咸湖卤虫幼体中较高的EPA本底含量有关。另外,两种单胞藻对两个品系卤虫的DHA含量没有显著提升作用,但破囊壶菌强化咸海卤虫幼体的DHA含量达到2.31 mg/g dw,这与破囊壶菌本身较高的DHA含量(115 mg/g dw)有关。本研究为高密度短期卤虫营养强化在水产育苗中的应用提供了数据参考。
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| 关 键 词： | 单胞藻 破囊壶菌 卤虫 营养强化 n-3 HUFA |
| 收稿时间： | 2023/1/16 0:00:00 |
| 修稿时间： | 2023/7/15 0:00:00 |
High-density and short-term nutritional enrichment of Artemia nauplii |
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| Gao Meirong,Deng Honggang,Xing Mingwei,Jia Jing,Zhou Guanghang,Long Feiping,Wu Xingyu,Wang Pian and Sui Liying.High-density and short-term nutritional enrichment of Artemia nauplii[J].Journal of Shanghai Ocean University,2024,33(2). |
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| Authors: | Gao Meirong Deng Honggang Xing Mingwei Jia Jing Zhou Guanghang Long Feiping Wu Xingyu Wang Pian and Sui Liying |
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| Abstract: | Short-term Artemia enrichment is an efficient approach for improving the desired nutritional value of live feed Artemia nauplii. To investigate the optimal enrichment conditions, newly-hatched Artemia nauplii were enriched with commercially produced dry powder of microalgae Phaeodactylum tricornutum and Isochrysis zhanjiangensis, and fugus Schizochytrium aggregatum in this study. And the n-3 HUFA enrichment with the above products were compared with two commercially important Artemia strains from Great Salt Lake, Utah, USA (GSL) and Aral Lake, Uzbekistan. The results showed that higher Artemia survival percentage (85~90%) were obtained when Artemia nauplii were enriched with I. zhanjiangensis under following conditions: enrichment dose (0.05, 0.1, 0.15 and 0.2 g/L), enrichment duration (12 h, 18 h and 24 h) and Artemia density (150, 200 and 250 ind./mL). And the individual body length and total biomass of Artemia increased with the prolonged duration and the increased dose. Considering the Artemia size preference and easy operation in the hatchery, the optimal enrichment parameters are proposed: Artemia nauplii density 150~200 ind./mL, enrichment dose 0.1~0.2 g/L, and enrichment duration 12~24 h. Fatty acid analysis showed that fatty acid enrichment in Artemia closely related to the enrichment agent and Artemia strains. For GSL Artemia, P. tricornutum and I. zhanjianggensis enrichment remarkably increased the EPA contents (7.39 and 3.20 mg/g DW, respectively) in Artemia when compared with newly-hatched Artemia nauplii (1.63 mg/g dw); whilst for Aral Artemia, slight increase of EPA content could be observed between the enriched-Artemia (17.59 mg/g DW and 17.26 mg/g DW, respectively) and newly-hatched Artemia nauplii (14.35 mg/g dw), showing that the enrichment effect closely related to the original EPA content of Artemia strains. On the other hand, the DHA content in two strains of Artemia did not show remarkable improvement when enriched with microalgae products, but S. aggregatum enrichment remarkably improved the DHA content of Aral Artemia (2.31 mg/g dw), which related to the high DHA content of S. aggregatum (115 mg/g dw). The results of this study provide the reference for application of Artemia nutrition enrichment in hatcheries. |
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| Keywords: | Microalgae Schizochytrium aggregatum Artemia Nutritional enrichment n-3 HUFA |
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