| 基于DNA条形码的如东海域浒苔附着鱼卵的物种鉴定 |
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| 引用本文： | 陈月华,何培民,杨金权.基于DNA条形码的如东海域浒苔附着鱼卵的物种鉴定[J].上海海洋大学学报,2018,27(1):1-7. |
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| 作者姓名： | 陈月华 何培民 杨金权 |
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| 作者单位： | 上海海洋大学,上海海洋大学,上海海洋大学水产种质资源发掘与利用教育部重点实验室 |
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| 基金项目： | 国家自然科学基金资助(31172066) |
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| 摘 要： | 为了探明如东海域浒苔藻团中附着的大量鱼卵的产卵鱼种,利用DNA条形码技术对其进行了物种鉴定。鉴定结果表明:鱼卵、仔鱼、成鱼与沙氏下鱵鱼(Hyporhamphus sajori)COI基因片段序列之间无变异位点出现,遗传距离为0,而与其他颌针鱼目鱼类序列间差异达10.83%~20.94%,遗传距离在21.9%~26.4%之间;NJ分子系统发育分析显示鱼卵、仔鱼、成鱼与沙氏下鱵鱼聚为单系群。因此,确定该海域浒苔藻团中产卵鱼类为沙氏下鱵鱼。在此基础上探讨了浒苔藻团附着大量黏性鱼卵现象的原因及其对近海鱼类资源恢复的启示。
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| 关 键 词： | DNA条形码 沙氏下鱵鱼 浒苔 鱼类资源恢复 |
| 收稿时间： | 2017/3/15 0:00:00 |
| 修稿时间： | 2017/10/11 0:00:00 |
Molecular identification of fish eggs in Enteromorpha of Rudong sea area based on DNA barcode |
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| CHEN Yuehu,HE Peimin and YANG Jinquan.Molecular identification of fish eggs in Enteromorpha of Rudong sea area based on DNA barcode[J].Journal of Shanghai Ocean University,2018,27(1):1-7. |
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| Authors: | CHEN Yuehu HE Peimin and YANG Jinquan |
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| Institution: | 15692165260,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University |
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| Abstract: | To identify the fish eggs which were obtained from Enteromorpha algae of Rudong sea area, DNA barcoding technique was used. Our result showed there were no variable sites in mtDNA COI gene sequences among our fish eggs, larvaes, adults and Japanese halfbeak (Hyporhamphus sajori), and the genetic distance among them was 0. However, high variable sites among the eggs, larvaes, adults and the other Beloniformes fishes, and the genetic distance among them is 21.9%-26.4%. The Neighbor-joining(NJ) phylogenetic tree also indicated that the eggs, larvaes, adults and the Japanese halfbeak were formed a monophyletic group. Therefore, it is suggested that the fish eggs which we obtained from Enteromorpha algae of Rudong sea area to be Hyporhamphus sajori. Based on our identification result, we further discussed the reasons of Japanese halfbeak spawning in algae and how to use this phenomenon on recovery the offshore fish resources. |
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| Keywords: | DNA barcode Hyporhamphus sajori Enteromorpha Fish resources recovery |
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