| 一株猪源芽孢杆菌益生菌的筛选与鉴定 |
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| 引用本文： | 李刚,王世英,郭晓军,张德全,朱宝成.一株猪源芽孢杆菌益生菌的筛选与鉴定[J].湖北农业科学,2010,49(3). |
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| 作者姓名： | 李刚 王世英 郭晓军 张德全 朱宝成 |
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| 作者单位： | 1. 河北农业大学生命科学学院,河北保定,071001
2. 石家庄市动物防疫监督总站,石家庄,050000 |
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| 基金项目： | 石家庄市科学技术研究与发展计划项目 |
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| 摘 要： | 以大肠杆菌(Escherichia coli)为病源指示菌,从养猪场健康成年猪粪便中分离、筛选出8株对其有抑菌活性的芽孢杆菌。经培养特征、形态观察、生理生化试验以及16S rDNA序列分析,通过对照《常见细菌鉴定手册》确定L-7菌株为枯草芽孢杆菌(Bacillus subtilis),16S rDNA序列相似度为99.58%,是一株比较理想的潜在益生菌。
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| 关 键 词： | 猪 益生菌 筛选 鉴定 枯草芽孢杆菌 |
Screening and Identification of A Probiotic Strain of Bacillus from Swine Intestine |
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| Abstract: | Eight Bacillus strains with the antagonistic activities against Escherichia coli were isolated from the intestinal tract of healthy and adult swine. Their morphology characteristics, physiological, biochemical properties and 16S rDNA sequence were analyzed and the L-7 strain was primarily identified as Bacillus subtilis which referred to the manual of common identification of bacteria. The similarity of 16S rDNA sequence between the isolated and Bacillus subtilis was 99.58%. The results showed that this strain was an ideal potential probiotic. |
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| Keywords: | swine probiotic screening identification Bacillus subtilis |
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