Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device |
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| Authors: | Fabiana Aparecida Santilli Brandão Kele Amaral Alves Danielle Cristina Calado de Brito Leda Maria Costa Pereira Glayciane Bezerra de Morais Lucy Vanessa Sulca Ñaupas Samara Silva de Souza Benner Geraldo Alves Ana Paula Ribeiro Rodrigues Dárcio Ítalo Alves Teixeira |
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| Institution: | 1. Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil;2. Ovid Research Company, Berkeley, CA, USA;3. Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil |
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| Abstract: | The present study evaluated the effect of Ovarian Tissue Cryosystem (OTC) on follicular morphology and density, as well as on stromal cell density of vitrified canine ovarian tissue. Canine ovarian fragments collected from adult female dogs in stages of the random oestrous cycle were fixed (FC, fresh control) or vitrified (VIT) with an OTC device. After vitrification and warming, the fragments were fixed for histological analysis. Overall, the mean percentage of normal pre-antral follicles decreased after vitrification procedure (FC: 74.5% ± 1.6% vs. VIT: 52.05% ± 1.5%). Although the rates of normal primordial (71.1% ± 1.8%) and secondary (0.7% ± 0.4%) follicles vitrified showed a reduction (p < .05), vitrification using OTC showed considerable preservation of follicles, when compared to the fresh control (81.1% ± 1.5% and 2.3% ± 0.6%, respectively). The mean follicular density was maintained after vitrification (FC: 199.65 ± 12.8 vs. VIT: 199.68 ± 10.8), whereas the stromal cell density decreased in the VIT group. Based on the results, we recommend the use of OTC for vitrification of canine ovarian tissue. |
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| Keywords: | bitches cryopreservation pre-antral follicles preservation of fertility |
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