| 香蕉茎尖的玻璃化法超低温保存及其植株再生 |
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| 引用本文： | 吴黎明,曾继吾,彭抒昂,易干军,周碧容,吴元立.香蕉茎尖的玻璃化法超低温保存及其植株再生[J].园艺学报,2006,33(3):501-505. |
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| 作者姓名： | 吴黎明 曾继吾 彭抒昂 易干军 周碧容 吴元立 |
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| 作者单位： | (广东省农业科学院果树研究所, 广东广州510640;华中农业大学园艺林学学院, 湖北武汉430070) |
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| 摘 要： | 以香蕉(Musa spp. ) 为试材, 对其离体培养茎尖玻璃化法超低温保存影响因素进行研究。结果表明, 不定芽在MS + 3.0～5.0 mg/L 6-BA + 0.1 mg/L NAA的培养基上分化较好。香蕉茎尖超低温保存较佳体系是: 2.0～3.0 cm的茎尖在含0.4 mol/L蔗糖培养基上预培养2 d, 剥取带1～2个叶原基的茎尖(长1.0～1.5 mm) , 室温(25℃) 下装载液(MS + 2 mol/L甘油+ 0.4 mol/L蔗糖) 装载20～30 min, 然后用玻璃化溶液( PVS2 ) 于0℃下处理40 min, 换1次PVS2后迅速投入液氮。保存至少1 h后, 在40℃水浴中化冻90 s, 用1.2 mol/L蔗糖培养液洗涤2次, 每次10 min, 然后转入含0.3 mol /L蔗糖的MS培养基上,暗培养10～15 h后转移到含0.5 mg/L 62BA的MS培养基中, 暗培养1周后转移到正常光下, 3个香蕉品种(巴西蕉、广东香蕉2 号、广东粉蕉1 号) 的成活率分别为75.9%、40.0%和69.6% , 再生率分别为63.4%、35.0%和63.4%。再生植株生长和分化正常, 生根后可移栽成活。
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| 关 键 词： | 香蕉 茎尖 超低温保存 玻璃化法 |
| 文章编号： | 0513-353（2006）03-0501-06 |
| 收稿时间： | 2005-06-11 |
| 修稿时间： | 2005-06-112005-10-02 |
Cryopreservation of in Vitro Shoot Tips of Banana by Vitrification and Its Regeneration |
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| Wu Liming,Zeng Jiwu,Peng Shuang,Yi Ganjun,Zhou Birong,Wu Yuanli.Cryopreservation of in Vitro Shoot Tips of Banana by Vitrification and Its Regeneration[J].Acta Horticulturae Sinica,2006,33(3):501-505. |
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| Authors: | Wu Liming Zeng Jiwu Peng Shuang Yi Ganjun Zhou Birong Wu Yuanli |
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| Institution: | Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of ;Science and Technology, Wuhan 430022, China |
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| Abstract: | Shoot tips excised from healthy in vitro plants of three Banana cultivars were successfully cryopreserved with the vitrification technique. A suitable procedure was established as follows: 3 - 4 weeks after subculture, shoot tip s about 2 - 3 cm in length were precultured for 2 days onMS medium supplemented with0.4 mol/L sucrose. Dissected shoot apices about 1.0 - 1.5 mm in length with one or two leaf primordium were loaded with a mixture of 2 mol/L glycerol plus 0.4 mol/L sucrose for 20 - 30 min at room temperature. These excised shoot tips were sufficiently dehydrated in a highly concentrated plant vitrification solution for 40 min at 0℃. And then plunged directly into liquid nitrogen and conserved for at least 1 h. After rapid thawing in water at 40℃ for 90 s, the tips were rinsed with 1.2 mol/L sucrose solution for 20 min at room temperature and then plated on MS medium supplemented with 0.5 mg/L 6-BA. The cryopreserved materials were cultured in darkness for one week so that they could grow quickly. Successfully cryopreserved shoot tips resumed growthwithin 10 days of plating and developed shootswithin 1 month without intermediary callus formation. This simple protocol was successfully applied to the three cultivars belonging to two genomic group s of banana. Thesurvival rate of shoot tips was 75.9% , 40.0% , 69.6% , and regrowth rate reached 63.4% , 35.0% , 63.4% respectively. Almost all the shoot tips formed roots and were successfully transferred to soil in pots. The plantlets could normally root and survive after transplantation. |
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| Keywords: | Banana Shoot-tip Cryopreservation Vitrification |
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