| ‘丰香’草莓叶片高效再生体系的建立 |
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| 引用本文： | 秦永华,张上隆,徐凯,吴延军,秦巧平.‘丰香’草莓叶片高效再生体系的建立[J].园艺学报,2005,32(1):101-104. |
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| 作者姓名： | 秦永华 张上隆 徐凯 吴延军 秦巧平 |
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| 作者单位： | (浙江大学园艺系, 农业部园艺植物生长发育与生物技术重点开放实验室, 杭州310029; 安徽农业大学园艺系, 合肥230036) |
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| 基金项目： | 国家自然科学基金项目(30170648) |
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| 摘 要： | 以草莓主栽品种丰香( Fragaria ×ananassa Duch ‘Toyonoka’) 叶片为外植体, 研究了影响组织培养的多个因素, 建立了高效再生体系。结果表明: MS + TDZ 1.5 mg·L - 1 + IBA 0.4 mg·L - 1培养基最适于不定芽分化。不同滤光膜(绿膜、红膜、蓝膜、黄膜) 对草莓不定芽的分化具显著效应, 绿膜和红膜对芽的分化有明显促进作用, 不定芽再生率达95%以上, 平均每个外植体再生芽数在25个以上; 而蓝膜和黄膜则不利于芽的分化。不同滤光膜光谱差异主要集中在300～700 nm, 红、绿膜在该波段光强较弱,而黄、蓝膜和荧光灯较强。暗处理4周比1、2、3周更有利于提高叶片的不定芽再生率, 此后转到光下培养, 获得的不定芽再生率可达100%。
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| 关 键 词： | 草莓 滤光膜 不定芽再生 暗处理 |
| 文章编号： | 0513-353X(2005)01-0101-04 |
| 收稿时间： | 2004-03-09 |
A Highly Efficient System Establishment of Shoot Regeneration from Leaf Explants of Strawberry |
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| Qin Yonghua,Zhang Shanglong,Xu Kai,Wu Yanjun,Qin Qiaoping.A Highly Efficient System Establishment of Shoot Regeneration from Leaf Explants of Strawberry[J].Acta Horticulturae Sinica,2005,32(1):101-104. |
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| Authors: | Qin Yonghua Zhang Shanglong Xu Kai Wu Yanjun Qin Qiaoping |
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| Institution: | ( Department of Horticulture, Zhejiang University, The Ministry of Agricultural Laboratory of Horticultural Plant Growth, Development and Biotechnology, Hangzhou 310029, China; Department of Horticulture, Anhui Agricultural University, Hefei 230036, China) |
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| Abstract: | An efficient and reliable method for shoot regeneration from leaf disks of strawberry (Fragaria ananassa Duch ‘Toyonoka’) was established. The optimum medium for shoot regeneration was MS supplemented with thidiazuron ( TDZ) 1.5 mg·L - 1 and Indole-3-butyric acid ( IBA) 0-4 mg·L - 1. Different color films had great effect on the shoot regeneration of strawberry. The presence of red and green films induced the maximum percentage of shoot regeneration ( > 95% ) and the highest number of buds per explant( > 25). However, the blue and yellow films inhibited the shoot regeneration of strawberry. The difference of films spectrum was focused on 300 - 700 nm. The light intensity of red and green films was weaker than yellow, blue and white ones. Dark treatment could effectively p romote shoot regeneration rate. In contrast, maximum percentage of shoot regeneration (100% ) and the highest number of buds per explant (9.05) were observed with dark treatment of four weeks. |
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| Keywords: | Strawberry Color plastic films System of regeneration Dark treatment |
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