| 乳链菌肽抗性嗜热链球菌的筛选 |
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| 引用本文： | 焦世耀,张兰威,韩建春,李春霖,于贵军.乳链菌肽抗性嗜热链球菌的筛选[J].东北农业大学学报,2005,36(1):66-70. |
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| 作者姓名： | 焦世耀 张兰威 韩建春 李春霖 于贵军 |
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| 作者单位： | 东北农业大学食品学院,黑龙江,哈尔滨,150030;黑龙江北亚乳业有限公司,黑龙江,大庆,163316 |
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| 摘 要： | 采用两倍稀释法在脱脂乳培养基和乳清培养基中测定了乳链菌肽(Nisin)对嗜热链球菌9的最小抑菌浓度(MIC)。在脱脂乳培养基中逐渐增加Nisin的浓度,驯化嗜热链球菌9,直到其抗性达到300IU·mL-1。经生产性能鉴定和遗传性状实验,获得两株适宜做酸奶发酵剂的Nisin抗性嗜热链球菌9.31和9.4。
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| 关 键 词： | 乳链菌肽 抗性菌株筛选 嗜热链球菌 |
| 文章编号： | 1005-9369(2005)01-0066-05 |
| 修稿时间： | 2004年2月27日 |
Isolation of Nisin-resistant streptococcus thermophilus |
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| JIAO Shi-yao,ZHANG Lan-wei,HAN Jian-chun,LI Chun-lin,YU Gui-jun.Isolation of Nisin-resistant streptococcus thermophilus[J].Journal of Northeast Agricultural University,2005,36(1):66-70. |
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| Authors: | JIAO Shi-yao ZHANG Lan-wei HAN Jian-chun LI Chun-lin YU Gui-jun |
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| Institution: | JIAO Shi-yao1,ZHANG Lan-wei1,HAN Jian-chun1,LI Chun-lin2,YU Gui-jun2 |
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| Abstract: | The MIC of Nisin to Streptococcus thermophilus are determined by serial twofold dilutions in skim milk medium and whey medium. In skim milk medium, Streptococcus thermophilus 9 was tamed by gradually increasing the Nisin concentration until its resistance attain 300 IU·mL-1 of Nisin per mL. The Nisin-resistant Streptococcus thermophilus 9.31 and 9.4 that fit for the yogurt culture were gained by production performance identification and genetic stability test. |
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| Keywords: | Nisin isolation Nisin-resistant Streptococcus thermophilus |
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