| 小麦甲基结合蛋白基因MBD3重组表达载体的构建和原核表达 |
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| 引用本文： | 孟凡荣,司志飞,刘昊英,张问.小麦甲基结合蛋白基因MBD3重组表达载体的构建和原核表达[J].中国农学通报,2008,24(11):78-80. |
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| 作者姓名： | 孟凡荣 司志飞 刘昊英 张问 |
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| 作者单位： | 河南农业大学生命科学学院,郑州,450002 |
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| 摘 要： | 本研究构建了小麦甲基结合蛋白基因MBD3的原核表达载体pGEX-4T-MBD3, 转化大肠杆菌BL21 (DE3)工程菌株，在37 ºC，1 mM IPTG浓度条件下，成功诱导表达了的GST-MBD3融合蛋白，大小为49.6 kDа，这为进一步开展MBD3的蛋白纯化和功能分析奠定了基础。
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| 关 键 词： | 肉牛 肉牛 三元杂交 增重 |
| 收稿时间： | 2008-08-12 |
| 修稿时间： | 2008-09-08 |
Recombinant expression vector with wheat MBD3 gene and its prokaryotic expression |
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| Meng Fanrong,Si Zhifei,Liu Haoying,Zhang Wen.Recombinant expression vector with wheat MBD3 gene and its prokaryotic expression[J].Chinese Agricultural Science Bulletin,2008,24(11):78-80. |
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| Authors: | Meng Fanrong Si Zhifei Liu Haoying Zhang Wen |
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| Abstract: | In this study, a prokaryotic expression vector pGEX-4T-MBD3 for the wheat MBD (Methyl-binding domain protein) gene MBD3 was constructed. Transform it to E. coli. BL21 (DE3), the fusion protein GST-MBD3 with molecular weight of 49.6 kDа was effectively expressed under 1mM IPTG concentrations at 37°C, which provides a foundation for further purification and functional study of the MBD3 protein. |
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| Keywords: | MBD3 vector construction prokaryotic expression |
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