| 山羊皱胃平滑肌细胞的培养及鉴定 |
| |
| 引用本文： | 邵春艳,徐瑞,赵佳,尉鹏,李建基.山羊皱胃平滑肌细胞的培养及鉴定[J].中国奶牛,2010(1):43-45. |
| |
| 作者姓名： | 邵春艳 徐瑞 赵佳 尉鹏 李建基 |
| |
| 作者单位： | 扬州大学兽医学院,扬州,225009 |
| |
| 摘 要： | 用外科手术方法取山羊皱胃的平滑肌组织,采用组织块培养的方法,在体外进行山羊皱胃平滑肌细胞的分离培养,为反刍动物皱胃疾病的研究提供了实验材料。结果表明,本研究成功培养出山羊皱胃的平滑肌细胞。倒置显微镜下,细胞生长状态良好,呈现平滑肌细胞特征性的"峰-谷"状结构,活细胞达95%以上,免疫组化染色显示细胞浆内平滑肌肌动蛋白呈阳性表达。
|
| 关 键 词： | 皱胃平滑肌细胞 分离 培养 鉴定 |
Culture and Identify of Goats Abomasul Smooth Muscle Cells in Vitro |
| |
| Shao Chunyan,Xu Rui,Zhao Jia,Wei Peng,Li Jianji.Culture and Identify of Goats Abomasul Smooth Muscle Cells in Vitro[J].China Dairy Cattle,2010(1):43-45. |
| |
| Authors: | Shao Chunyan Xu Rui Zhao Jia Wei Peng Li Jianji |
| |
| Institution: | Shao Chunyan,Xu Rui,Zhao Jia,Wei Peng,Li Jianji (School of Veterinary Medicine,Yangzhou University,Yangzou 225009) |
| |
| Abstract: | In the study, surgical methods was used to take goat abomasum of the smooth muscle tissues. Using tissue culture methods, we isolated and cultured the goats abomasul smooth muscle cells in vitro in order to provide experimental material for ruminants abomasum disease study. The Results showed that goats abomasul smooth muscle cells were cultured successfully in vitro. From inverted microscope, we can observed that cell growth were in good condition, and showed characteristic smooth muscle cells of thepeak-v... |
| |
| Keywords: | Abomasums Smooth muscle cells Isolate Culture Identify |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
