| 丰产接骨木茎段离体快繁的研究 |
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| 引用本文： | 赵学彩,赵红霞,王凯,刘欣,李媛媛,吴海涛.丰产接骨木茎段离体快繁的研究[J].中国农学通报,2018,34(7):53-57. |
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| 作者姓名： | 赵学彩 赵红霞 王凯 刘欣 李媛媛 吴海涛 |
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| 作者单位： | 山东博华高效生态农业科技有限公司,山东博华高效生态农业科技有限公司,山东博华高效生态农业科技有限公司,山东博华高效生态农业科技有限公司,山东博华高效生态农业科技有限公司,山东博华高效生态农业科技有限公司 |
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| 基金项目： | 山东省科技重大专项（重大关键技术）“耐盐碱林木及经济作物品种选育及示范推广”(2015ZDJS03003)。 |
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| 摘 要： | 为了加快接骨木良种的快繁,以接骨木优良株系的扦插苗为实验材料进行接骨木组织培养研究,建立其再生体系。结果表明：外植体的最佳消毒方法为75%酒精消毒30s加 0.1%Hgcl2消毒15min,成活率80%；诱导腋芽的最优培养基为MS NAA 0.05mg/L,诱导率98.7%,增殖培养基为MS 6-BA1mg/L TDZ0.01mg/L,增殖系数5.0；生根培养基1/2MS IBA0. 1mg/L,生根率100%；移栽时最佳基质,珍珠岩：草炭土=3:5,成活率95%。
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| 关 键 词： | 短时强降水 短时强降水 高分辨率 分布特征 |
| 收稿时间： | 2017/3/6 0:00:00 |
| 修稿时间： | 2017/4/19 0:00:00 |
Tissue Culture Research on Stem Segment of High-yielding Sambucus williamsii |
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| Abstract: | The tissue culture system of high-yielding Sambucus williamsii was established using young stem segment of cutting seedlings as the material, aimed to speed up the breeding of improved varieties. The results showed that the proper method for surface sterilization was treating the stem segment of cutting seedlings with 75% alcohol and 0.1% HgCl2 for 30 s and 15 min, and the survival rate could reach 80%. The medium suitable for bud germination was MS+ NAA 0.05 mg/L, with the induction rate of 98.7%. The optimal culture medium for multiplication was MS+ 6-BA 1.0 mg/L+ TDZ 0.01 mg/L+ NAA 0.1 mg/L, with multiplication coefficient of 3.82. The rooting medium was 1/2MS+ IBA 0.1 mg/L, with the best growth and the rooting rate of 100%. When transplanted, a combination of perlite and humus in the ratio of 3:5 was adopted as the medium, and the survival rate could reach over 95%. |
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| Keywords: | Sambucus williamsii stems with axillary bud tissue culture |
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