| 3种芹菜茎组织总RNA提取方法的比较研究 |
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| 引用本文： | 董姻然,陈湘宁,常希光,黄健.3种芹菜茎组织总RNA提取方法的比较研究[J].安徽农业科学,2014(8):2273-2275,2314. |
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| 作者姓名： | 董姻然 陈湘宁 常希光 黄健 |
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| 作者单位： | 北京农学院食品科学与工程学院,北京102206;农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206;北京市优质农产品产销服务站,北京,100101 |
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| 基金项目： | 现代农业产业技术体系北京市叶类蔬菜创新团队. |
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| 摘 要： | 目的]为了获得质量较好的芹菜茎的总RNA,为后续分子生物学试验提供基础。方法]试验对1种RNA提取方法(Trizol法)和2种RNA提取试剂盒(RNA prep Pure Plant Kit法、RNA simple Total RNA Kit法)方法进行比较研究。结果]RNA prepPure Plant Kit方法得率低,且OD260/OD280值低于1.8,Trizol法OD260/OD230高于2.0,且得率低于RNA simple Total RNA kit法。只有RNA simple Total RNA kit法提取的RNA质量最好,得率最高。结论]与Trizol法和RNA prep Pure Plant Kit法相比,RNA simple Total RNA Kit法提取的芹菜茎总RNA得率高,完整性好,凝胶电泳泳道无杂质,可以满足半定量RT-PCR的试验需要。
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| 关 键 词： | 芹菜 总RNA提取 RNA simple Total RNA Kit法 |
The Comparison of Several Kinds of Methods for Extracting Total RNA from the Stalk of Celery |
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| Institution: | DONG Yin-ran, CHEN Xiang-ning et al ( Department of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206; Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Beijing 102206) |
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| Abstract: | Objective ] In order to obtain better quality total RNA in the stalk of celery and lay the foundation for subsequent molecular biology experiments. Method] One RNA extraction method（ Trizol method） and two RNA extraction kits （RNA prep Pure Plant Kit method, RNA simple Total RNA Kit method） were compared. Result] The yield of RNA prepPure Plant Kit is low, OD260/OD280 value is lower than 1.8, OD260/OD230 of Trizol method is higher than 2.0, the yield is lower than RNA simple Total RNA kit method. RNA extracted by RNA simple Total RNA kit has the best quality and the highest yield. Conclusion] The results showed that the Tfizol method was better than two RNA extraction kit method, the total RNA isolated with Trizol method had high integrity, high yield, good stability, and no pollution in the gel electrophoresis track. And then could meet the needs of the semi-quantitative RT-PCR test. |
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| Keywords: | Celery Total RNA extraction RNA simple Total RNA Kit method |
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