| 一株纤维素降解菌的鉴定 |
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| 引用本文： | 解玉红,葛艳辉,曹杨,贾记红,强艳艳.一株纤维素降解菌的鉴定[J].安徽农业科学,2008,36(24). |
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| 作者姓名： | 解玉红 葛艳辉 曹杨 贾记红 强艳艳 |
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| 作者单位： | 天津理工大学环境科学与安全工程学院,天津,300191 |
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| 基金项目： | 天津市科委资助项目，天津市高校科技发展计划项目 |
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| 摘 要： | 目的]鉴定已筛选出的纤维素降解菌,选育性能优良的产纤维素酶菌株。方法]在固体培养基中培养产纤维素酶菌株K7-2,观察其群体形态、个体形态,并从生理生化特性、分子生物学方面对其进行鉴定。结果]K7-2菌落为白色,表面光滑、干燥致密,呈绒布状,边缘整齐,在培养基中呈辐射状生长,揭开培养皿可闻到土腥味。K7-2菌体为丝状,革兰氏染色阳性,插片培养可见分生孢子丝和分生孢子。在菌株K7-2群体形态、个体形态和生理生化特性鉴定的基础上,通过16sDNA测序鉴定,确定K7-2为梅久兰链霉菌(Streptomycesmediolani)。结论]该研究为选育高产纤维素酶菌株提供了理论依据。
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| 关 键 词： | 纤维素酶 梅久兰链霉菌 鉴定 |
Identification of Degrading Bacteria of One Plant of Cellulase |
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| XIE Yu-hong et al.Identification of Degrading Bacteria of One Plant of Cellulase[J].Journal of Anhui Agricultural Sciences,2008,36(24). |
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| Authors: | XIE Yu-hong |
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| Abstract: | Objective] The aim was to identify the screened cellulose degrading bacteria,breed the strains producing cellulases with high quality performance.Method] The strains producing cellulases of K7-2 were cultured in solid medium,and their population shape and individual form were observed and identified from the aspects of physiological and biochemical characteristics and molecular biology.Result] The K7-2 colonies were white with smooth surface,dry and compact,presented a flocculent state and trim edge,and grew in radiated shape in medium,the earthy odor could be smelled when the culture dish was opened.K7-2 thalli was in thread shape,with gram-positive,and the conidium hyphae and conidia could be seen in plate insert culture.On the basis of identification of the population shape,individual form and physiological and biochemical characteristics of strain K7-2 which was determined as Streptomyces mediolani through 16sDNA sequence appraisal.Conclusion] The experimental basis was provided for selection and breeding of high yield strains of cellulose by this research. |
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| Keywords: | Cellulase Streptomyces mediolani Identification |
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