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单瓣和重瓣花中国水仙的核型比较
引用本文:任艳蕊,刘传斌,李晓峰.单瓣和重瓣花中国水仙的核型比较[J].河北农业大学学报,2010,33(2).
作者姓名:任艳蕊  刘传斌  李晓峰
作者单位:1. 河北农业大学,园艺学院,河北,保定,071001
2. 保定市农业科学研究所,河北,保定,071000
摘    要:以中国水仙(Narcissus tazetta var.chinensis)为材料,研究了不同预处理对根尖细胞中期分裂指数的影响,并对单瓣和重瓣花中国水仙的核型进行了比较和分析。结果表明:用α-溴萘饱和水溶液在活体条件下处理根尖4 h,中期细胞的分裂指数最高,且染色体分散、清晰;单瓣和重瓣花中国水仙均为同源三倍体;单瓣花的核型公式为2n=3x=30=6m+6sm+18st(3SAT),核型类型为3C;重瓣花为2n=3x=30=6sm+24st(6SAT),核型类型为进化程度更高的4C。

关 键 词:中国水仙  核型  染色体组

Comparison of karyotype between univalve Chinese narcissus and multiple-valve Chinese narcissus
REN Yan-rui,LIU Chuan-bin,LI Xiao-feng.Comparison of karyotype between univalve Chinese narcissus and multiple-valve Chinese narcissus[J].Journal of Agricultural University of Hebei,2010,33(2).
Authors:REN Yan-rui  LIU Chuan-bin  LI Xiao-feng
Institution:1.College of Horticulture;Agricultural University of Hebei;Baoding 071001;China;2.Agricultural Institute of Baoding;Baoding 071000;China
Abstract:Chinese narcissus (Narcissus tazetta var. chinensis) was used as material,The effect of different pre-treatments on cell division index of Chinese narcissus (Narcissus tazetta var. chinensis) root cell division at matephase was studied,and the karyotypes of univalve flower Chinese narcissus and multiple-valve flower Chinese narcissus were analyzed and compared. The results showed that the best method was treating the roots in vivo with saturated liquid of α- bromomaphthalene for 4 hours,the index of cell division at metaphase reached at 7 85%. Both kinds of Chinese narcissus are triploid. The karyotype formula of univalve flower Chinese narcissus is 2n=3x=30=6m+6sm +18st(3SAT) and belongs to 3C;the karyotype formula of multiple valve flower Chinese narcissus is 2n=3x=30=6sm +24st(6SAT) and belongs to 4C.
Keywords:Chinese narcissus  karyotype  genome  
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