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Enhancement of resistance to soft rot (Pectobacterium carotovorum subsp. carotovorum) in transgenic Brassica rapa |
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| Authors: | Young-Jin Koh Jong-In Park Nasar Uddin Ahmed Hee-Jeong Jung Kwon-Kyoo Kang Yoonkang Hur Yong-Pyo Lim Ill-Sup Nou |
| Institution: | 1. Department of Plant Medicine, Sunchon National University, Suncheon, 540-742, Republic of Korea 2. Department of Horticulture, Sunchon National University, Suncheon, 540-742, Republic of Korea 3. Department of Horticulture, Hankyong National University, Ansung, 456-749, Republic of Korea 4. Department of Biology, Chungnam National University, Daejeon, 305-764, Republic of Korea 5. Department of Horticulture, Chungnam National University, DaeJeon, 305-764, Republic of Korea |
| Abstract: | Brassica rapa (chinese cabbage) is one of the main vegetable crops grown in Asian countries. Pectobacterium carotovorum subsp. carotovorum causes severe economic loss in this crop as well as in other Brassica crops through soft rot disease. Cysteine proteases like bromelain, papain or ficin show toxic effects to herbivorous insects and pathogenic bacteria. They have been known to be critical factors in plant defence mechanisms. The current study investigated the effect of bromelain gene (BL1) of pineapple (Ananas comosus L. Merrill) on enhancing resistance to soft rot in transgenic Brassica rapa ‘Seoulbaechu’. Three homozygous T2 lines were inoculated with Pectobacterium carotovorum subsp. carotovorum and BL8-2 line showed the lowest rate of infected leaves (RIL) in both wound inoculation and non-wound inoculation, when the non-infected line showed 100 % RIL in both cases. The highest expression of BL1 gene was also observed in BL8-2 homozygous line. Thus, the over-expressed BL1 gene conferred enhanced resistance to soft rot in Brassica rapa. |
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