| 小鼠扩张囊胚低渗液处理试验 |
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| 引用本文： | 朱士恩.小鼠扩张囊胚低渗液处理试验[J].中国畜牧杂志,1998,34(2):9-10. |
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| 作者姓名： | 朱士恩 |
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| 作者单位： | 中国农业大学动物科技学院 |
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| 摘 要： | 为研究解冻后的胚胎膨胀对其发育率的影响，本试验用２０～５０％的低渗ＰＢＳ溶液（即０．２０×、０．２５×、０．３０×及０．５０×），分别将小鼠扩张囊胚在低渗液中处理３０分钟后培养，结果新鲜胚在０．５０×和０．３０×中处理后的发育率分别为１００％和９１％，０．２０×处理组的发育率降低到５３％。解冻后的胚胎直接用低渗液处理，０．５０×组只有８５％继续发育，低于０．２５×组完全不能发育。而解冻后经培养恢复到扩张囊胚后再做低渗处理，０．３０×组仍有９６％继续发育。
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| 关 键 词： | 低渗液 膨胀 玻璃化 扩张囊胚 小鼠 |
Study on Expanded Mouse Blastocysts Treated with Lower Osmatic Solution |
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| Zhu Shien\ Zeng Shenming\ Zhang Zhongcheng.Study on Expanded Mouse Blastocysts Treated with Lower Osmatic Solution[J].Chinese Journal of Animal Science,1998,34(2):9-10. |
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| Authors: | Zhu Shien\ Zeng Shenming\ Zhang Zhongcheng |
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| Institution: | College of Animal Science and Technology China Agricultural University Beijing 100094 |
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| Abstract: | Expanded mouse blastocyst were treated with lower osmotic solution,20～50% PBS(0.20x,0.25x,0.30x and 0.50x),for 30min to study effects of expanding in thawed embryos on development. Fresh embryos treated with 0.50x,0.30x and 0.20x PBS resulted in development rates of 100%,91% and 53% respectively. Thawde embryos directly treated with 0.50x PBS got developmental rate of 85%,but couldn′t develop with 0.25x PBS. If thawed embryos were re expanded to blastocysts in mKRB firstly,then treated with lower osmatic PBS,the development rates were still 96% in the group treated with 0.30x PBS. |
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| Keywords: | Lower osmatic solution\ Expand\ Vitrification\ Expanded blastocyst\ Mouse |
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