| 小麦新品种“成电麦1号”α-醇溶蛋白基因的分离与序列分析 |
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| 引用本文： | 李光蓉,郎涛,刘成,周建平,任正隆,杨足君.小麦新品种“成电麦1号”α-醇溶蛋白基因的分离与序列分析[J].中国农学通报,2011,27(1):203-208. |
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| 作者姓名： | 李光蓉 郎涛 刘成 周建平 任正隆 杨足君 |
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| 作者单位： | 电子科技大学生命科学与技术学院,成都,610054 |
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| 基金项目： | 国家自然科学基金项目，教育部新世纪人才计划项目，四川省小麦育种攻关项目 |
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| 摘 要： | 以染色体工程方法培育小麦新品种“成电麦1号”基因组DNA为模板，用小麦种子醇溶蛋白保守引物进行PCR扩增。扩增产物经克隆测序，获得978-1310 bp的6个序列（CD-1、CD-9、CD-11、CD-12、CD-17和CD-21），其中3个序列（CD-1、CD-12、CD-17）编码284~301个氨基酸的α-醇溶蛋白基因，所编码的氨基酸序列在多聚谷氨酰胺区和重复区出现较大差异，在编码序列的半胱氨酸的数量和位置上，CD-1和CD-12序列具有特殊性。与乳糜泻（celiac disease）病人毒性抗原相关序列的比对结果表明CD-12不具有Glia-α20表位，而CD-1和CD-17序列只具有Glia-α9，Glia-α20的抗原序列。根据已发表的小麦A、B或D染色体组的α-醇溶蛋白基因的序列建立系统树，发现所克隆的“成电麦1号”α-醇溶蛋白基因序列具有特殊性，说明染色体工程方法培育的小麦新品种具有较大的种子蛋白基因变异，这些基因对小麦品质育种的作用值得进一步研究。
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| 关 键 词： | 多模型 多模型 农产品 供求信息 预测 |
| 收稿时间： | 6/7/2010 12:00:00 AM |
| 修稿时间： | 7/9/2010 12:00:00 AM |
Isolation and Sequence Analysis of α-gliadin Genes from Wheat Cultivar‘Chengdianmai 1’ |
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| Li Guangrong,Lang Tao,Liu Cheng,Zhou Jianping,Ren Zhenglong,Yang Zujun.Isolation and Sequence Analysis of α-gliadin Genes from Wheat Cultivar‘Chengdianmai 1’[J].Chinese Agricultural Science Bulletin,2011,27(1):203-208. |
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| Authors: | Li Guangrong Lang Tao Liu Cheng Zhou Jianping Ren Zhenglong Yang Zujun |
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| Institution: | Li Guangrong,Lang Tao,Liu Cheng,Zhou Jianping,Ren Zhenglong,Yang Zujun ( School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054) |
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| Abstract: | Based on the conserved regions of known α -gliadin genes, gene-specific primers for α -gliadin were designed to amplify the full gene coding regions from the DNA of wheat cultivar ‘ Chengdianmai 1 ’ , which was developed by wheat-Secale wide cross and chromosome manipulation. The PCR products were cloned and sequenced. Total 6 sequences were obtained with length of 978 to 1310 bp, in which CD-1, CD-9, CD-11, CD-12, CD-17 and CD-12 were 1207 bp, 978 bp, 1310 bp, 1258 bp, 1210 bp, 1218 bp, respectively. Three of them were full ORF sequences （CD-1, CD-12 and CD-17）, containing the coding regions of 284~301 amino acids, and the other three were pseudo genes. Multi-alignment analysis indicated that three full ORF sequences had higher similarity with other α -gliadin genes, and the differences mainly existed in the two polyglutamine regions and the repetitive domains. CD-12 is lack of the Glia- α 20 epitope, whereas CD-1 and CD-17 only contained the Glia- α 9 and Glia- α 20 epitopes. For the two polyglutamine regions, length of sequences was strongly different and some glutamines were replaced by other residues. The coposition and number of the cystenin residue of the sequences indicated that the CD-1 and CD-12 exhibited unique characters comparing with the other reported α -gliadin genes. The phylogenetic tree indicated that all the full α -gliadin gene ORF sequences from ‘ Chengdianmai ’ 1 could not be clustered into the group of α -gliadin genes from the A, B and D genomes of wheat （Triticum aestivum）, but presented in a new group, indicating the great gliadin genes divergence during the breeding program of chromosome engineering. The role of these genes in wheat quality and breeding needed to be further studied. |
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| Keywords: | Triticum aestivum α -gliadin gene gene cloning |
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