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| IP3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员 | |
| 引用本文： | 李洋洋,杜希华,于涌鲲,赵福宽,孙清鹏.IP3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员[J].中国农学通报,2011,27(1):149-153. |
| 作者姓名： | 李洋洋 杜希华 于涌鲲 赵福宽 孙清鹏 |
| 作者单位： | 1. 山东师范大学生命科学学院,济南,250014;北京农学院生物技术学院,北京,102206 2. 山东师范大学生命科学学院,济南,250014 3. 北京农学院生物技术学院,北京,102206 |
| 基金项目： | 国家自然科学基金"环核苷酸门控的离子通道在拟南芥茉莉酸信号通路中的作用"、"AtCNGC2/AtCNGC19在茉莉酸诱导的钙离子动员中的分子机制"，北京市自然科学基金"AtCNGC离子通道在茉莉酸诱导的钙动员中的作用"，北京市科技新星计划"AtCNGC2或AtCNGC4在茉莉酸诱导的钙离子动员中的作用" |
| 摘 要： | 以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中，利用LAS AF（Leica Application Suite-Advanced Fluorescence）软件记录肝素对茉莉酸（JA）诱导的胞内钙离子荧光强度的变化。结果显示，经不同浓度的肝素预处理后，拟南芥叶细胞中胞内钙离子的荧光强度降低，再用100 μmol/L JA处理时，其荧光强度升高，但仅与未经肝素处理的荧光强度相当。实验证明，肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。 |
| 关 键 词： | 拟南芥 钙离子 肝素 茉莉酸 |
| 收稿时间： | 2010/6/22 0:00:00 |
| 修稿时间： | 8/5/2010 12:00:00 AM |
IP3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization |
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| Li Yangyang,Du Xihua,Yu Yongkun,Zhao Fukuan,Sun Qingpeng.IP3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization[J].Chinese Agricultural Science Bulletin,2011,27(1):149-153. | |
| Authors: | Li Yangyang Du Xihua Yu Yongkun Zhao Fukuan Sun Qingpeng |
| Institution: | Li Yangyang 1,2,Du Xihua 1,Yu Yongkun 2,Zhao Fukuan 2,Sun Qingpeng 2 ( 1 College of Life Sciences,Shandong Normal University,Jinan 250014,2 College of Biotechnology,Beijing University of Agriculture,Beijing 102206) |
| Abstract: | Arabidopsis thaliana leaves were labeled by fluorescent probe Fluo-3/AM under low temperature at 4℃ to measure the fluorescent intensity of intracellular Ca 2 ＋ which was pretreated with heparin on jasmonic acid （JA）-induced. The results showed that the fluorescent intensity of Ca 2 ＋ ] cyt was reduced after pretreated with different concentration of heparin, and then treated with 100 μmol/L JA, the fluorescent intensity of Ca 2 ＋ ] cyt was close to the fluorescent intensity which was not pretreated with heparin. The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca 2＋ concentration significantly which JA-induced in leaves of Arabidopsis thaliana. |
| Keywords: | Arabidopsis thaliana Ca 2 heparin jasmonic acid |
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