| 四倍体菘蓝基因组DNA提取方法的比较 |
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| 引用本文： | 段英姿,客绍英,马艳芝,张胜珍,马作东.四倍体菘蓝基因组DNA提取方法的比较[J].安徽农业科学,2010,38(17):8869-8870,8885. |
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| 作者姓名： | 段英姿 客绍英 马艳芝 张胜珍 马作东 |
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| 作者单位： | 唐山师范学院生命科学系,河北唐山,063000;唐山师范学院生命科学系,河北唐山,063000;唐山师范学院生命科学系,河北唐山,063000;唐山师范学院生命科学系,河北唐山,063000;唐山师范学院生命科学系,河北唐山,063000 |
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| 摘 要： | 目的]探讨四倍体菘蓝组培苗高质量DNA的提取方法。方法]分别采用CTAB法1、CTAB法2、SDS法1、SDS法2和碱裂解法对四倍体菘蓝组培苗DNA进行提取试验,通过紫外分光光度法和琼脂糖凝胶电泳,比较了5种DNA提取方法对四倍体菘蓝组培苗的提取效果。结果]CTAB法2提取DNA的OD260/OD230值为2.311,OD260/OD280值为1.774,DNA纯度最大,浓度最大,提取率最高,PCR扩增条带清晰,重复性好,所得DNA的质量最好。结论]CTAB法2是提取四倍体菘蓝组培苗基因组DNA最有效的方法。
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| 关 键 词： | 四倍体菘蓝 DNA 提取 CTAB法 SDS法 |
The Comparative Study of Genomic DNA Extraction Methods for the Tetraploid Isatis |
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| Institution: | DUAN Ying-zi et al(Department of Biology,Tangshan Teacher＇s College,Tangshan,Hebei 063000) |
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| Abstract: | Objective]The aim was to research the method of high-quality DNA extraction from leaves tissue culture of the tetraploid Isatis.Method]The extraction effects of five different DNA-extracting methods such as CTAB method 1,CTAB method 2,SDS Act 1,SDS France 2 and the alkaline lysis method were compared by the UV spectrophotometry and agarose gelelectrophoresis.Result]The results showed that CTAB method 2 was the best method,OD260/OD230 value was 2.311,OD260/OD280 value was 1.774,DNA was the most pure,and it had the largest concentration was the highest rate of extraction and the best DNA gua lity,PCR bands amplified clear and reproducible.Conclusion]CTAB method 2 was an effective way for DNA extraction from leaves tissue culture of the teproducible Isatis. |
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| Keywords: | DNA |
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