| 一株对美国白蛾高毒力苏云金芽孢杆菌的杀虫基因鉴定 |
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| 引用本文： | 董明,杜立新,王容燕,王金耀,曹伟平,宋健,冯书亮.一株对美国白蛾高毒力苏云金芽孢杆菌的杀虫基因鉴定[J].中国农学通报,2010,26(3):242-244. |
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| 作者姓名： | 董明 杜立新 王容燕 王金耀 曹伟平 宋健 冯书亮 |
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| 作者单位： | 1. 河北大学生命科学学院,河北保定,071000;河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定,071000
2. 河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定,071000 |
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| 基金项目： | 国家863计划项，国家科技支撑项目 |
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| 摘 要： | 美国白蛾是一种重要的检疫害虫,为进行有效地控制,筛选出了一株对其具有高毒力的苏云金芽孢杆菌。HYW-8是从黑龙江伊春市土壤中通过抗生素筛选法分离到的一株形成小菱形晶体的苏云金芽孢杆菌,利用PCR-RFLP对其基因型进行鉴定,并采用浸液法对美国白蛾进行生物活性测定。结果发现,菌株HYW-8含有cry1Ab、cry1E基因和一种未知cry2基因。生物测定结果表明,其对美国白蛾具有很高的杀虫毒力,LC_(50)为7.4×10~5芽孢/mL,低于标准菌株HD-1。因此,HYW-8对防治美国白蛾具有较强的应用潜力。
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| 关 键 词： | 高温平菇 高温平菇 农艺性状 评价 |
| 收稿时间： | 2009-11-04 |
| 修稿时间： | 2009-11-23 |
Characterization of One Bacillus thuringiensis Strain Highly Toxic to Hyphantria cunea |
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| Dong Ming,Du Lixin,Wang Rongyan,Wang Jinyao,Cao Weiping,Song Jian,Feng Shuliang.Characterization of One Bacillus thuringiensis Strain Highly Toxic to Hyphantria cunea[J].Chinese Agricultural Science Bulletin,2010,26(3):242-244. |
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| Authors: | Dong Ming Du Lixin Wang Rongyan Wang Jinyao Cao Weiping Song Jian Feng Shuliang |
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| Institution: | Dong Ming~(1,2),Du Lixin~2,Wang Rongyan~2,Wang Jinyao~2,Cao Weiping~2,Song Jian~2,Feng Shuliang~2 (1 College of Life Sciences,Hebei University,Baoding Hebei 071000,2 Institute of Plant Protection,Hebei Academy of Agricultural , Forestry Sciences,IPM Centre of Hebei Province,Baoding Hebei 071000) |
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| Abstract: | Hyphantria cunea was an important quarantine pest.In this study,we selected a Bacillus thuringiensis strain with high virulence for the biocontrol of Hyphantria cunea.The strain HYW-8 was isolated from the soil in the city of Yichun by antibiotic method.It produced small diamond-shaped crystals.It was identified that contained cry1A,cry1E gene and an unknown cry2 gene by PCR-RFLP.Bioassay showed that the strain HYW-8 had high insecticidal activity to Hyphantria cunea.The LC50 was 7.4×10~5 spore/mL,which was lower than the standard strain of Bt subsp kurstaki strain HD-1.We suggested that the potential of the strain HYW-8 for used in microbial control of the larvae of Hyphantria cunea. |
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| Keywords: | PCR-RFLP |
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