| 玉米秸秆还田土壤中纤维素分解菌的分离筛选和鉴定（摘要） |
| |
| 引用本文： | 郭艳,张进良,王会平,齐辉,梁峰.玉米秸秆还田土壤中纤维素分解菌的分离筛选和鉴定（摘要）[J].农业科学与技术,2010,11(4):134-137,147. |
| |
| 作者姓名： | 郭艳 张进良 王会平 齐辉 梁峰 |
| |
| 作者单位： | 郭艳,梁峰(商丘师范学院生命科学系,河南商丘,476000;河南省高校生物质降解与气化工程技术研究中心,河南商丘,476000);张进良,王会平,齐辉(商丘师范学院生命科学系,河南商丘,476000) |
| |
| 基金项目： | 河南省教育厅自然基金，河南省高校科技创新人才支持计划，大型秸秆生物气化装置关键技术研究与示范，大型级联式消化秸秆沼气发生装置关键技术研究与示范(2009A180013).Supported by Natural Foundation of Education Department of Henan Province，Science and Technology Innovation Supporting Program Talents of University in Henan Province，Key Technology Research and Demonstration of Large-scale Straw Gasification Equipment，Key Technology Research and Demonstration of Large Cascade Straw Digestion Biogas Generating Installation |
| |
| 摘 要： | 目的]从玉米秸杆还田土壤中分离筛选出酶活力较高的纤维素分解苗。方法]通过CMC固体培养初筛和摇床培养复筛从玉米秸秆还田土壤中筛选出纤维素酶活力较高的菌株,并对其进行16rDNA序列分析。结果]通过初筛和复筛获得了内切酶活力较高的1株细菌和1株真菌,滤纸酶活力较高的1株真菌和2种酶活均较高的1株细菌（5号菌株）。16SrDNA序列分析结果表明,5号菌株与Bacillus subtilis的相似性达到100%。结论]5号菌株鉴定为枯草芽炮杆菌
|
| 关 键 词： | 秸秆还田 纤维素分解菌 筛选 纤维素酶 |
Isolation, Screening and Identification of Cellulose Decomposing Strains in Straw-amended Soil |
| |
| GUO Yan,ZHANG Jin-liang,WANG Hui-ping,QI Hui,LIANG Feng.Isolation, Screening and Identification of Cellulose Decomposing Strains in Straw-amended Soil[J].Agricultural Science ＆ Technology,2010,11(4):134-137,147. |
| |
| Authors: | GUO Yan ZHANG Jin-liang WANG Hui-ping QI Hui LIANG Feng |
| |
| Institution: | 1.Department of Life Science,Shangqiu Normal University,Shangqiu 476000;2.Engineering Technology Research Center of Biomass Degradation and Gas-forming,University of Henan Province,Shangqiu 476000 ) |
| |
| Abstract: | Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium（NO.5 strain） with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.Conclusion]NO.5 strain was identified as Bacillus subtilis. |
| |
| Keywords: | Straw-amended soil Cellulose decomposing strains Screening Cellulase |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
